The high-resolution X-ray structure of the adduct formed upon the reaction of hen egg white lysozyme (HEWL) with the potential drug BMOV (bis(maltolato)oxidovanadium(IV) or [V^IVO(malt)₂], where malt = maltolato) from crystals grown in 1.1 M NaCl and 0.1 M sodium acetate at pH 4.0 reveals an unexpected reaction product, where the V compound is transformed, the maltolate ring is broken and a significant modification of the N-terminal lysine side chain is observed. The results, also supported by gel electrophoresis and mass spectrometry data, show an unusual reactivity of BMOV with HEWL in the solid state, prompting further research on the reactivity of vanadium compounds with proteins which could depend on the specific experimental conditions.

中文翻译：

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潜在药物双（麦芽糖）氧化钒（IV）与溶菌酶的晶体反应性出乎意料


鸡蛋清溶菌酶（HEWL）与潜在药物BMOV（双（麦芽酸）氧化钒（IV）或[V ^IV O（麦芽）反应形成的加合物的高分辨率X射线结构) ₂ ]，其中麦芽=麦芽糖)，在 pH 4.0 的 1.1 M 氯化钠和 0.1 M 乙酸钠中生长的晶体显示出意想不到的反应产物，其中 V 化合物被转化，麦芽糖酸酯环被破坏，并且观察到 N 末端赖氨酸侧链的显着修饰。这些结果也得到了凝胶电泳和质谱数据的支持，表明 BMOV 与 HEWL 在固态下具有不寻常的反应性，这促使人们进一步研究钒化合物与蛋白质的反应性，这可能取决于具体的实验条件。