, a commonly cultivated tree, exhibits leaflet nyctinastic movement. However, the underlying mechanism remains unclear. The pulvinus, as a motor organ, coordinates the motion of its attached leaflet. During the leaflet expansion, flexor cells shift from contraction to expansion, with concurrent expansion and fusion of vacuole. In contrast, the extensor cells move from expansion to contraction, causing vacuole contraction and increased vacuole numbers. During the process of leaflet expansion and closure, the contents of IAA and IPA in flexor muscles increased significantly, but did not show significant changes in extensor muscles, BR content showed an opposite trend in extensor and flexor muscles, and the content of ZR increased significantly in extensor muscles but decreased significantly in flexor muscles, and ZR content only decreased in extensor muscles, but did not show significant changes in flexor muscles. Exogenous IAA (50–100 μM) significantly aids in leaflet unfolding during night time. Transcriptome analysis reveals gene enrichment in circadian rhythms, hormone signaling, ion channels, and other pathways during leaflet opening and closure. The expression of , and is prominent during expansion, while stands out during closure. These findings suggest a potential regulatory pathway in 'Daguo Tianyangtao 2′ leaflets, involving light, circadian rhythm, hormone signaling, and ion channels. Findings of this study can do a great deal to broaden the plant nyctinastic movement research.

中文翻译：

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杨桃小叶夜色中激素调控及候选基因的探索


，一种常见栽培的树，表现出小叶夜间运动。然而，其根本机制仍不清楚。枕叶作为运动器官，协调其附着的小叶的运动。在小叶扩张期间，屈肌细胞从收缩转变为扩张，同时液泡扩张和融合。相反，伸肌细胞从扩张移动到收缩，导致液泡收缩和液泡数量增加。在小叶扩张和闭合过程中,屈肌中IAA和IPA含量显着增加,而伸肌中未出现明显变化,伸、屈肌中BR含量呈现相反趋势,ZR含量显着增加ZR含量仅在伸肌中下降，而在屈肌中显着下降，ZR含量仅在伸肌中下降，而在屈肌中没有表现出明显变化。外源 IAA (50–100 μM) 显着有助于夜间小叶展开。转录组分析揭示了小叶打开和关闭过程中昼夜节律、激素信号传导、离子通道和其他途径的基因富集。 、 和 的表达在展开时突出，而在闭合时突出。这些发现表明“大果天养桃2号”小叶中存在潜在的调控途径，涉及光、昼夜节律、激素信号传导和离子通道。这项研究的结果可以极大地拓宽植物夜间运动的研究。