The possibility of application of poly(4-vinyl pyridine) layers cross-linked with transition metal complexes as active layers in biomedical sensors was tested. The successful modification of the P4VP coating with CuBr or ZnBr was verified using time of flight - secondary ion mass spectrometry and X-ray photoelectron spectroscopy. The topography and wettability of the coatings were examined by using atomic force microscopy and water contact angles measurements, respectively. Tests of biological activity of coatings indicated strong protein adsorption, good biocompatibility, and no antimicrobial activity. The potential of the coatings to be used as active layers of biosensors was verified, by systematic impedance-based measurements, which showed the sensitivity of the P4VP:CuBr coatings to the presence of proteins and cells in different concentrations. The high selectivity of the coatings toward the defined analyte was confirmed by the specific antigen–antibody immunoreaction, and the possibility of monitoring of protein adsorption and cell adhesion also for individual cells was presented. Finally, the conductive response of a bilayer system that mimics Organic Field Effect Transistor was shown. These results point to a great potential for both coatings to serve as active layers of sensitive and highly selective biosensors.

中文翻译：

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与过渡金属配合物交联的聚（乙烯基吡啶）涂层作为对蛋白质吸附和细胞粘附敏感的生物传感器的活性层


测试了与过渡金属配合物交联的聚（4-乙烯基吡啶）层作为生物医学传感器中的活性层的应用的可能性。使用飞行时间二次离子质谱和 X 射线光电子能谱验证了 CuBr 或 ZnBr 对 P4VP 涂层的成功修饰。分别通过原子力显微镜和水接触角测量来检查涂层的形貌和润湿性。涂层生物活性测试表明，其蛋白质吸附能力强，生物相容性好，且无抗菌活性。通过基于阻抗的系统测量验证了该涂层用作生物传感器活性层的潜力，该测量显示了 P4VP:CuBr 涂层对不同浓度的蛋白质和细胞的存在的敏感性。特异性抗原抗体免疫反应证实了涂层对特定分析物的高选择性，并且提出了监测单个细胞的蛋白质吸附和细胞粘附的可能性。最后，显示了模仿有机场效应晶体管的双层系统的导电响应。这些结果表明这两种涂层作为敏感和高选择性生物传感器的活性层具有巨大的潜力。