The quality of traditional Chinese medicine (TCM) is the prerequisite for ensuring its safe and effective clinical application. With the increasing popularity of TCM worldwide, the quality control of TCM products has become increasingly crucial. Tianma toutong tablet (TMTTT) is mainly used for migraine caused by external wind and cold, blood stasis, or deficiency of blood and nourishment. However, the mechanism of action of TMTTT is still unclear, and there has been a lack of in vitro antioxidant activity research and migraine treatment mechanism research. Therefore, it is urgent to establish a set of comprehensive and effective evaluation methods. three fingerprint profiles were established using HPLC, UV, and DSC analysis methods, and established three digital parameters simple complexity index (), simple clarity index (), simple complexity clarity ratio (), 22 batches of samples were evaluated using a comprehensive linear quantitative fingerprint method (CLQFM). In addition, the antioxidant activity of the samples was determined using the DPPH method, and the relationship between fingerprint peaks in different fingerprints and antioxidant capacity was explored using Pearson correlation coefficients. Finally, network pharmacological research was conducted to investigate the potential targets, compounds, and pathways involved in the treatment of migraines with TMTTT. The results showed that the 22 batches of samples were classified into different quality grades. TMTTT exhibited good antioxidant activity, the fingerprint-efficacy relationship showed that gastrodin, chlorogenic acid, ferulic acid, imperatorin and isoimperatorin had strong antioxidant capacity, providing directions for the identification of active compounds. A total of 36 core targets were identified and screened by network pharmacology, which AKT serine/threonine kinase 1 (AKT1), albumin (ALB), insulin (INS), tumor necrosis factor (TNF), prostaglandin-endoperoxide synthase 2 (PTGS2) and interleukin-6 (IL-6), and compounds such as β-sitosterol, chrysophanol, vanillin are the key to the treatment of migraine, providing references for subsequent clinical research and new drug development. Significance: This study examined the consistency of the quality of TMTTT and the mechanism of action in treating migraines from both quality and efficacy perspectives, providing a favorable direction for further research on TMTTT and offering new ideas for the quality control of TCM compound formulations.

中文翻译：

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利用指纹技术和网络药理学从质量和功效双重维度综合研究天麻头痛片


中药质量是保证临床应用安全有效的前提。随着中药在全球范围内的日益普及，中药产品的质量控制变得越来越重要。天麻头痛片（TMTTT）主要用于外感风寒、瘀血或血养两虚所致的偏头痛。但TMTTT的作用机制尚不清楚，且缺乏体外抗氧化活性研究和偏头痛治疗机制研究。因此，迫切需要建立一套全面、有效的评价方法。采用HPLC、UV、DSC分析方法建立了3个指纹图谱，并建立了简单复杂度指数（）、简单澄清度指数（）、简单复杂度净度比（）三个数字参数，采用综合线性定量法对22批样品进行评价指纹法（CLQFM）。此外，采用DPPH法测定样品的抗氧化活性，并利用Pearson相关系数探讨不同指纹图谱中的指纹峰与抗氧化能力的关系。最后，进行了网络药理学研究，以探讨 TMTTT 治疗偏头痛的潜在靶点、化合物和途径。结果显示，22批次样品划分为不同质量等级。 TMTTT表现出良好的抗氧化活性，指纹图谱-药效关系表明天麻素、绿原酸、阿魏酸、欧前胡素和异欧前胡素具有较强的抗氧化能力，为活性化合物的鉴定提供了方向。 通过网络药理学共鉴定筛选出36个核心靶点，其中AKT丝氨酸/苏氨酸激酶1（AKT1）、白蛋白（ALB）、胰岛素（INS）、肿瘤坏死因子（TNF）、前列腺素内过氧化物合酶2（PTGS2）和白细胞介素6（IL-6）以及β-谷甾醇、大黄酚、香草醛等化合物是治疗偏头痛的关键，为后续临床研究和新药研发提供参考。意义：本研究从质量和疗效两个角度考察了TMTTT的质量一致性及其治疗偏头痛的作用机制，为TMTTT的进一步研究提供了有利的方向，也为中药复方制剂的质量控制提供了新思路。