The coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in widespread disease transmission, challenging the stability of global healthcare systems. Surface-enhanced Raman scattering (SERS) as an easy operation, fast, and low-cost technology illustrates a good potential in detecting SARS-CoV-2. In the study, one-step fabrication of gold-silver alloy nanoparticles (AuAgNPs) with adjustable metal proportions and diameters is employed as SERS substrates. The angiotensin-converting enzyme 2 (ACE2) functionalized AuAgNPs are applied as sensor surfaces to detect SARS-CoV-2 S protein. By optimizing the SERS substrates, ACE2/AuAgNPs illustrate higher performance in detecting the SARS-CoV-2 S protein with a limit of detection (LOD) of 10 fg/mL in both phosphate-buffered saline (PBS) and pharyngeal swabs solution (PSS). It also provides excellent reproducibility with a relative standard deviation (RSD) of 7.7 % and 7.9 %, respectively. This easily preparable and highly reproducible SERS substrate has good potential in the practical application of detecting SARS-CoV-2.

中文翻译：

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一步合成多尺寸金银合金纳米颗粒，具有高 SERS 灵敏度，可直接检测 SARS-CoV-2 刺突蛋白


由严重急性呼吸综合征冠状病毒 2 (SARS-CoV-2) 引起的 2019 冠状病毒病 (COVID-19) 已导致疾病广泛传播，对全球医疗保健系统的稳定性构成挑战。表面增强拉曼散射 (SERS) 作为一种操作简单、快速且成本低廉的技术，在检测 SARS-CoV-2 方面具有良好的潜力。在这项研究中，采用一步法制造具有可调节金属比例和直径的金银合金纳米粒子（AuAgNPs）作为SERS基底。血管紧张素转换酶 2 (ACE2) 功能化的 AuAgNPs 被用作传感器表面来检测 SARS-CoV-2 S 蛋白。通过优化 SERS 底物，ACE2/AuAgNP 在检测 SARS-CoV-2 S 蛋白方面表现出更高的性能，在磷酸盐缓冲盐水 (PBS) 和咽拭子溶液 (PSS) 中的检测限 (LOD) 均为 10 fg/mL ）。它还具有出色的重现性，相对标准偏差 (RSD) 分别为 7.7% 和 7.9%。这种易于制备且重复性高的SERS底物在检测SARS-CoV-2的实际应用中具有良好的潜力。