Friction has been considered to mediate physiological activities of cells, however, the biological friction between a single cell and its ligand-bound surface has not been thoroughly explored. Herein, we established a friction model for single cells based on an atomic force microscopy (AFM) combined with an inverted fluorescence microscopy (IFM) to study the friction between a highly sensitive platelet and fibrinogen-coated surface. The study revealed that the friction between the platelet and fibrinogen-coated tip is mainly influenced by specific ligand–receptor interaction. Further, we modeled the biological friction, which consists of specific interaction, non-specific interaction, and mechanical effect. Besides, the results suggested that the velocity can also affect specific ligand–receptor interactions, resulting in the friction change and platelet adhesion to fibrinogen surfaces. The study built a friction model between a single cell and its ligand-bound surface and provided a potential method to study the biological friction by the combination of AFM and IFM.

摩擦被认为介导细胞的生理活动，然而，单个细胞与其配体结合表面之间的生物摩擦尚未得到彻底探索。在此，我们建立了基于原子力显微镜（AFM）与倒置荧光显微镜（IFM）相结合的单细胞摩擦模型，以研究高度敏感的血小板和纤维蛋白原涂层表面之间的摩擦。研究表明，血小板和纤维蛋白原涂层尖端之间的摩擦主要受特定配体-受体相互作用的影响。此外，我们模拟了生物摩擦，其中包括特异性相互作用、非特异性相互作用和机械效应。此外，结果表明速度还会影响特定的配体-受体相互作用，导致摩擦力变化和血小板对纤维蛋白原表面的粘附。该研究建立了单细胞与其配体结合表面之间的摩擦模型，为结合AFM和IFM研究生物摩擦提供了一种潜在的方法。