Microbiologically influenced corrosion (MIC) is a type of localized corrosion caused by the metabolic activity of microorganisms and is responsible for at least 10–20% of incidents in the oil and gas industry. Therefore, researchers are focused on technologies to prevent MIC. In this study, gamma radiation using a Cobalt-60 (Co) source was applied to the microbial community present in oil-field produced water samples obtained from conventional (COP) and unconventional (UOP) oil processes. Doses of 5, 10, 15, and 20 kGy were used. The total bacterial concentration and the efficiency of the treatment were determined as a function of the dose and the time after irradiation through ATP quantification. The content of specific genes was quantified through qPCR. In addition, microbial diversity was monitored through metagenomic study of the bacterial 16S gene. Total bacteria, sulfate-reducing bacteria (SRB), and sp. were quantified in COP water samples. Meanwhile, total bacteria, SRB, thiosulfate-reducing bacteria, and sp. were quantified in UOP water samples. The irradiation process was highly efficiency for the microbiological control in COP and UOP water samples. A decrease in the microbial load of at least one logarithm was obtained for all the samples. A greater decrease in cell concentration was observed after 15 and 30 days of irradiation, with a greater decay (>90%) at higher doses administered. Molecular analysis revealed that bacteria such as sp., sp., sp., and sp. showed a decreasing trend with increasing irradiation doses rates. The findings of this investigation suggest that the application of ionizing radiation may enhance the efficacy of microbial treatment in produced waters in the oil and gas industry.

中文翻译：

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电离辐射对阿根廷常规和非常规石油生产中产出水中微生物的影响


微生物影响腐蚀 (MIC) 是一种由微生物代谢活动引起的局部腐蚀，是石油和天然气行业中至少 10-20% 事故的原因。因此，研究人员将重点放在预防MIC的技术上。在这项研究中，使用钴 60 (Co) 源的伽马辐射应用于从传统 (COP) 和非常规 (UOP) 石油加工过程中获得的油田采出水样品中存在的微生物群落。使用的剂量为 5、10、15 和 20 kGy。通过 ATP 定量，将总细菌浓度和处理效率确定为辐射后剂量和时间的函数。通过qPCR对特定基因的含量进行定量。此外，通过细菌 16S 基因的宏基因组研究监测微生物多样性。细菌总数、硫酸盐还原菌 (SRB) 和 sp。在 COP 水样中进行了定量。同时，细菌总数、SRB、硫代硫酸盐还原菌和 sp。在 UOP 水样中进行了定量。辐照过程对于 COP 和 UOP 水样的微生物控制非常有效。所有样品的微生物负荷均减少了至少一个对数。照射 15 天和 30 天后观察到细胞浓度下降幅度更大，施用剂量较高时细胞浓度下降幅度更大 (>90%)。分子分析表明，细菌如 sp.、sp.、sp. 和 sp.。随辐照剂量率的增加呈下降趋势。这项调查的结果表明，电离辐射的应用可以提高石油和天然气工业采出水中微生物处理的效果。