The nuclear factor Y (NF–Y) is a class of heterotrimeric transcription factors comprising three subunits: NF-YA, NF-YB, and NF-YC. These transcription factors participate in many plant bioprocesses, including the regulation of flowering time. Although the gene family has been systematically studied in many species, little is known about its role in the non-heading Chinese cabbage (NHCC) [ (syn) ssp. ]. In this study, we identified 57 NF–Y members in the genome of NHCC using BLASTP, including 20 , 24 , and 13 . These genes are randomly distributed on the 10 chromosomes of NHCC. The results of yeast two hybrid experiments indicated that among some members of the three subunits of BcNF-Ys, the members of the NF-YA and NF-YC subunits interact with each other, a third of the members of the NF-YB and NF-YC subunits interact with each other, while no interaction was observed between the members of the NF-YA and NF-YB subunits. Subcellular localization experiments in tobacco showed that BcNF-YA2 and BcNF-YA8 were expressed in the nucleus; BcNF-YB18 and BcNF-YB23 were located in the cell membrane and cytoplasm; and BcNF-YC6 and BcNF-YC7 were expressed in the nucleus, cytoplasm, and cell membrane. We analyzed the -acting elements in the promoter of genes and found that the ABA response element is the most distributed hormone response element, which is regulated by ABA signals triggered by environmental stimuli. Accordingly, we treated three-week-old NHCC leaves with 100 μmol L ABA and analyzed the expression profile of s through RNA-seq. The results showed that except for six undetected , the remaining 51 showed varying degrees of response to ABA signals. Among these, was positively regulated by ABA signals, with the highest upregulation amplitude. Subsequently, the function of was extensively studied, which demonstrated that its expression promotes plant flowering. This result enriches our understanding of the potential molecular mechanism by which ABA positively regulates NHCC flowering.

中文翻译：

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不结球白菜 NF-Y 基因家族的全基因组分析以及 BcNF-YA8 在 ABA 介导的开花调控中的作用


核因子 Y (NF-Y) 是一类异源三聚体转录因子，包含三个亚基：NF-YA、NF-YB 和 NF-YC。这些转录因子参与许多植物生物过程，包括开花时间的调节。尽管该基因家族已在许多物种中进行了系统研究，但对其在不结球大白菜 (NHCC) [ (syn) ssp. 中的作用知之甚少。 ]。在本研究中，我们使用BLASTP鉴定了NHCC基因组中的57个NF-Y成员，包括20、24和13。这些基因随机分布在NHCC的10条染色体上。酵母二杂实验结果表明，BcNF-Ys 三个亚基的部分成员中，NF-YA 和 NF-YC 亚基的成员相互作用，NF-YB 和 NF 亚基的成员中有三分之一是相互作用的。 -YC 亚基彼此相互作用，而 NF-YA 和 NF-YB 亚基成员之间没有观察到相互作用。烟草亚细胞定位实验表明BcNF-YA2和BcNF-YA8在细胞核中表达； BcNF-YB18和BcNF-YB23定位于细胞膜和细胞质； BcNF-YC6和BcNF-YC7在细胞核、细胞质和细胞膜中表达。我们分析了基因启动子中的作用元件，发现ABA反应元件是分布最多的激素反应元件，它受到环境刺激触发的ABA信号的调节。因此，我们用 100 μmol L ABA 处理三周大的 NHCC 叶子，并通过 RNA-seq 分析 s 的表达谱。结果显示，除6个未检测到外，其余51个对ABA信号表现出不同程度的反应。其中，受ABA信号正向调节，上调幅度最高。 随后，其功能被广泛研究，证明其表达可促进植物开花。这一结果丰富了我们对 ABA 正向调节 NHCC 开花的潜在分子机制的理解。