Common methods based on enzymatic approaches for uric acid (UA) have potential issues such as limited detection range, low linearity, and susceptibility to interference. Our study focuses on enhancing the sensitivity and selectivity of detecting UA by decorating carbon nanotubes (CNTs) with bimetallic platinum-cobalt nanoparticles (PtCo/CNTs) using an adsorbing-thermal reducing method for electrochemical analysis. Physical characterizations are employed to confirm its structural and element compositions. Then, an electrochemical biosensor based on screen printed electrode (SPE) modified with PtCo/CNTs was constructed for UA determination. PtCo/CNTs modified SPE (PtCo/CNTs@SPE) demonstrates significant electrochemical activity and selectivity toward UA detection due to the synergistic effect between PtCo nanoparticles and CNTs, and the excellent electrical conductivity. Under optimized conditions, the current response of UA at the PtCo/CNTs@SPE presented linear dependence on the concentration, ranging from 0.1 µM to 3000.0 µM with a low limit of detection of 0.05 µM. In addition, the selectivity, stability, and practical applications of the as-prepared sensor are also explored.

中文翻译：

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双金属 PtCo 纳米颗粒修饰的碳纳米管用于灵敏检测尿酸


基于酶法的尿酸 (UA) 常用方法存在检测范围有限、线性度低和易受干扰等潜在问题。我们的研究重点是通过使用吸附热还原方法用双金属铂钴纳米粒子（PtCo/CNTs）装饰碳纳米管（CNTs）来提高检测UA的灵敏度和选择性，以进行电化学分析。采用物理表征来确认其结构和元素组成。然后，构建了一种基于 PtCo/CNT 修饰的丝网印刷电极（SPE）的电化学生物传感器，用于 UA 测定。由于 PtCo 纳米粒子和 CNT 之间的协同效应以及优异的导电性，PtCo/CNTs 改性 SPE (PtCo/CNTs@SPE) 表现出显着的电化学活性和对 UA 检测的选择性。在优化条件下，PtCo/CNTs@SPE 上 UA 的电流响应呈现出浓度的线性依赖性，范围从 0.1 µM 到 3000.0 µM，检测下限为 0.05 µM。此外，还探讨了所制备传感器的选择性、稳定性和实际应用。