AimTo investigate the relationship and potential causality between biological ageing and periodontitis.Materials and MethodsWe obtained the National Health and Nutrition Examination Survey (NHANES) and genome‐wide association study (GWAS) summary statistics as well as single‐cell sequencing data. Multivariate regression analysis based on cross‐sectional data, Mendelian randomization (MR) and multi‐omics integration analysis were employed to explore the causal association and potential molecular mechanisms between biological ageing and periodontitis. Additionally, two‐step MR mediation analysis explored the risk factors in biological ageing‐mediated periodontitis.ResultsWe analysed data from 3189 participants in the NHANES data and found that higher biological age was associated with increased risk of periodontitis. MR analyses revealed causal associations between biological age measures and periodontitis risk. Frailty (odds ratio [OR] = 2.08, 95% confidence interval [CI]: 1.04–4.18, p = .039) and GrimAge acceleration (OR = 1.16, 95% CI: 1.01–1.32, p = .033) were causally associated with periodontitis risk, and these results were validated in a large‐scale meta‐periodontitis GWAS dataset. Additionally, the risk effects of body mass index, waist circumference and lifetime smoking on periodontitis were partially mediated by frailty and GrimAge acceleration.ConclusionsEvidence from cross‐sectional survey and MR analysis suggests that biological ageing increases the risk of periodontitis. Additionally, improving the associated risk factors can help prevent both ageing and periodontitis.

中文翻译：

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生物衰老与牙周炎之间的关联：来自横断面调查和多组学孟德尔随机化分析的证据


目的探讨生物衰老与牙周炎之间的关系和潜在因果关系。材料和方法我们获得了全国健康与营养检查调查 （NHANES） 和全基因组关联研究 （GWAS） 汇总统计数据以及单细胞测序数据。采用基于横断面数据的多因素回归分析、孟德尔随机化 （MR） 和多组学整合分析来探讨生物衰老与牙周炎之间的因果关系和潜在的分子机制。此外，两步 MR 中介分析探讨了生物衰老介导的牙周炎的危险因素。结果我们分析了 NHANES 数据中 3189 名参与者的数据，发现较高的生物年龄与牙周炎风险增加相关。MR 分析揭示了生物年龄测量与牙周炎风险之间的因果关系。虚弱 （比值比 [OR] = 2.08,95% 置信区间 [CI]：1.04-4.18，p = .039）和 GrimAge 加速 （OR = 1.16,95% CI：1.01-1.32，p = .033） 与牙周炎风险有因果关系，这些结果在大规模元牙周炎 GWAS 数据集中得到了验证。此外，体重指数、腰围和终生吸烟对牙周炎的风险影响部分由虚弱和 GrimAge 加速介导。结论横断面调查和 MR 分析的证据表明，生物衰老会增加患牙周炎的风险。此外，改善相关的风险因素有助于预防衰老和牙周炎。