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A centrifugal-driven spiral microchannel microfiltration chip for emulsion and deformable particle sorting
Lab on a Chip ( IF 6.1 ) Pub Date : 2024-07-04 , DOI: 10.1039/d4lc00260a Yongchao Cai 1 , Zekun Li 1 , Cuimin Sun 2 , Xuan Zhao 1 , Shixiong Wu 1 , Guangyong Huang 3 , Shengchang Tang 1 , Peng Dai 1 , Xiangfu Wei 1 , Hui You 1
Lab on a Chip ( IF 6.1 ) Pub Date : 2024-07-04 , DOI: 10.1039/d4lc00260a Yongchao Cai 1 , Zekun Li 1 , Cuimin Sun 2 , Xuan Zhao 1 , Shixiong Wu 1 , Guangyong Huang 3 , Shengchang Tang 1 , Peng Dai 1 , Xiangfu Wei 1 , Hui You 1
Affiliation
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Droplet sorting and enrichment, as a prominent field within microfluidic technology, represent a pivotal stage in the manipulation of droplets and particles. In recent times, droplet sorting methods based on lab-on-disk (LOD) have garnered significant interest among researchers for their inherent merits, including high throughput, ease of operation, seamless device integration, and independence from supplementary driving forces. This study introduces a centrifugal force-driven microfluidic chip comprising spiral microchannels. The chip incorporates microhole arrays along the sidewall of the spiral channels, enabling size-based sorting and enrichment of microdroplets under the influence of multiple forces. Firstly, a comparative analysis was performed to assess the influence of the separation port structure and rotational speed on efficiency, and a mechanical modeling approach was employed to conduct kinetic analyses of droplet behavior during instantaneous separation. Those findings demonstrated a good agreement with the experimental results at ω < 100 rpm. Subsequently, sorting experiments on homogeneous droplets indicated that repetitive sorting could increase the recovery ratios, RT(α), of high-concentration droplets (20.7%) from 35.3% to over 80%. We also conducted a sorting experiment on three-component homogeneous-phase emulsions using a serially connected chip array, and the sorting throughput was 0.58 mL min−1. As a result, the RT(α) for 60 and 160 μm droplets were 99.4% and 88.9%, respectively. Lastly, we conducted elution experiments and dual-sample sorting on a single chip, and the fluorescence results demonstrated that this study provided an efficient and non-cross-contaminating sorting method for non-homogenous phase multi-sample microreactor units.
中文翻译：
一种用于乳液和可变形颗粒分选的离心驱动螺旋微通道微滤芯片
液滴分选和富集作为微流体技术中的一个突出领域，代表了液滴和颗粒操纵的关键阶段。近年来，基于磁盘实验室（LOD）的液滴分选方法因其固有优点而引起了研究人员的极大兴趣，包括高通量、易于操作、无缝设备集成以及独立于辅助驱动力。本研究介绍了一种由螺旋微通道组成的离心力驱动的微流控芯片。该芯片沿着螺旋通道的侧壁结合了微孔阵列，能够在多种力的影响下实现基于尺寸的微滴分类和富集。首先，通过比较分析评估分离口结构和转速对效率的影响，并采用机械建模方法对瞬时分离过程中的液滴行为进行动力学分析。这些发现与ω < 100 rpm 时的实验结果非常吻合。随后，对均质液滴的分选实验表明，重复分选可以将高浓度液滴（20.7％）的回收率RT（ α ）从35.3％提高到80％以上。我们还利用串联芯片阵列对三组分均相乳液进行了分选实验，分选通量为0.58 mL min -1 。结果，60和160μm液滴的RT( α )分别为99.4%和88.9%。 最后，我们在单芯片上进行了洗脱实验和双样品分选，荧光结果表明本研究为非均相多样品微反应器单元提供了一种高效且无交叉污染的分选方法。
更新日期：2024-07-09
中文翻译：
一种用于乳液和可变形颗粒分选的离心驱动螺旋微通道微滤芯片
液滴分选和富集作为微流体技术中的一个突出领域，代表了液滴和颗粒操纵的关键阶段。近年来，基于磁盘实验室（LOD）的液滴分选方法因其固有优点而引起了研究人员的极大兴趣，包括高通量、易于操作、无缝设备集成以及独立于辅助驱动力。本研究介绍了一种由螺旋微通道组成的离心力驱动的微流控芯片。该芯片沿着螺旋通道的侧壁结合了微孔阵列，能够在多种力的影响下实现基于尺寸的微滴分类和富集。首先，通过比较分析评估分离口结构和转速对效率的影响，并采用机械建模方法对瞬时分离过程中的液滴行为进行动力学分析。这些发现与ω < 100 rpm 时的实验结果非常吻合。随后，对均质液滴的分选实验表明，重复分选可以将高浓度液滴（20.7％）的回收率RT（ α ）从35.3％提高到80％以上。我们还利用串联芯片阵列对三组分均相乳液进行了分选实验，分选通量为0.58 mL min -1 。结果，60和160μm液滴的RT( α )分别为99.4%和88.9%。 最后，我们在单芯片上进行了洗脱实验和双样品分选，荧光结果表明本研究为非均相多样品微反应器单元提供了一种高效且无交叉污染的分选方法。
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