Super-resolution microscopy (SRM) is gaining popularity in biosciences; however, claims about optical resolution are contested and often misleading. In this Viewpoint, experts share their views on resolution and common trade-offs, such as labelling and post-processing, aiming to clarify them for biologists and facilitate deeper understanding and best use of SRM. In this Viewpoint, experts discuss resolution and common trade-offs in super-resolution microscopy, aiming to improve how biologists use the technology.

中文翻译：

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超分辨率显微镜中的分辨率——定义、权衡和观点


超分辨率显微镜（SRM）在生物科学领域越来越受欢迎；然而，有关光学分辨率的说法存在争议，而且常常具有误导性。在本观点中，专家们分享了他们对分辨率和常见权衡（例如标签和后处理）的看法，旨在为生物学家澄清这些问题，并促进对 SRM 的更深入理解和最佳利用。在本观点中，专家们讨论了超分辨率显微镜的分辨率和常见权衡，旨在改善生物学家使用该技术的方式。