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Exploring the landscape of miRNA production and the structural rules that shape it
Nature Plants ( IF 15.8 ) Pub Date : 2024-07-02 , DOI: 10.1038/s41477-024-01728-6


Degradome sequencing of semi-active DCL1 mutants separates true miRNAs from DCL1-independent small RNAs in Arabidopsis thaliana and illustrates the processing pattern of 147 pri-miRNAs. In parallel, DMS-MaPseq decodes the in vivo secondary structures of pri-miRNAs, enabling a better understanding of cleavage modes and of the impact of DCL1 cofactors on cleavage.

中文翻译:


探索 miRNA 生产的概况及其形成的结构规则



半活性 DCL1 突变体的降解组测序将拟南芥中真正的 miRNA 与不依赖 DCL1 的小 RNA 分开,并说明了 147 个 pri-miRNA 的加工模式。同时,DMS-MaPseq 解码 pri-miRNA 的体内二级结构,从而能够更好地了解切割模式以及 DCL1 辅因子对切割的影响。
更新日期:2024-07-02
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