Timely and accurate diagnosis is critical for effective healthcare, yet nearly half the global population lacks access to basic diagnostics. Point-of-care (POC) testing offers partial solutions by enabling low-cost, rapid diagnosis at the patient's location. At-home POC devices have the potential to advance preventive care and early disease detection. Nevertheless, effective sample preparation and detection methods are essential for accurate results. This review surveys recent advances in sample preparation and detection methods at POC. The goal is to provide an in-depth understanding of how these technologies can enhance at-home POC devices. Lateral flow assays, nucleic acid tests, and virus detection methods are at the forefront of POC diagnostic technology, offering rapid and sensitive tools for identifying and measuring pathogens, biomarkers, and viral infections. By illuminating cutting-edge research on assay development for POC diagnostics, this review aims to accelerate progress towards widely available, user-friendly, at-home health monitoring tools that empower individuals in personalized healthcare in the future.

中文翻译：

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面向未来家庭测试的护理点设备中的样品制备和检测方法


及时、准确的诊断对于有效的医疗保健至关重要，但全球近一半人口无法获得基本诊断。即时护理 (POC) 测试通过在患者所在地实现低成本、快速诊断来提供部分解决方案。家用 POC 设备有潜力促进预防保健和早期疾病检测。然而，有效的样品制备和检测方法对于获得准确的结果至关重要。本综述调查了 POC 样品制备和检测方法的最新进展。目标是深入了解这些技术如何增强家用 POC 设备。侧流检测、核酸检测和病毒检测方法处于 POC 诊断技术的前沿，为识别和测量病原体、生物标志物和病毒感染提供快速、灵敏的工具。通过阐明 POC 诊断检测开发的前沿研究，本综述旨在加速广泛可用、用户友好的家庭健康监测工具的进展，使个人在未来获得个性化医疗保健。