Background and ObjectivesRegulatory requirement of fixed holding time (6 h) of whole blood (WB) at room temperature, that is, 22–24°C (RT) results in sub‐optimal component separation. The aim was to evaluate the platelet concentrates (PC) prepared by both platelet rich plasma (PRP) and buffy coat (BC) methods after overnight hold (18–24 h) at RT.Materials and MethodsA prospective experimental study was performed. A total of 48 WB units collected were divided into four groups (12 each) control‐1 (C1) and test‐1 (T1) for PRP and control‐2 (C2) and test‐2 (T2) for the BC method. Control groups were processed within 6 h, and in test groups, components were prepared after overnight hold, followed by evaluation of quality parameters.ResultsIrrespective of the method used, all PCs had similar volume, platelet yield, swirling, no bacterial contamination, RBC contamination, PaO2 and PaCO2 levels. PCs in the T1 group had significant differences in glucose and MPV values on d1, which were resolved by d5 of storage. PCs in T2 has significant differences in pH, glucose, and MPV levels throughout storage. PRBC in test and control groups had similar quality parameters till d42 of storage. FFPs in all tests were noninferior to the concurrent control groups till 3 months of storage.ConclusionOvernight holding of WB had no lasting deleterious changes. Though a few biochemical parameters in the test groups were significantly different, they can be accepted to improve the logistics of component separation. Overall PRP method seemed to have a better result than the BC method after an overnight hold.

中文翻译：

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全血室温保存过夜（18-24 小时）后，对使用不同方法制备的血小板浓缩液进行评估


背景和目标全血 (WB) 在室温（即 22–24°C (RT)）下固定保存时间（6 小时）的监管要求会导致成分分离效果不佳。目的是评估在室温下过夜（18-24 小时）后通过富血小板血浆（PRP）和血沉棕黄层（BC）方法制备的血小板浓缩物（PC）。材料和方法进行了一项前瞻性实验研究。总共收集了 48 个 WB 单位，分为四组（每组 12 个），用于 PRP 的对照 1 (C1) 和测试 1 (T1)，以及用于 BC 方法的对照 2 (C2) 和测试 2 (T2)。对照组在 6 小时内处理，测试组在过夜后制备成分，然后评估质量参数。结果无论使用哪种方法，所有 PC 的体积、血小板产量、旋转、无细菌污染、红细胞污染均相似, 氧分压2和二氧化碳分压2水平。 T1组中的PC在第1天的葡萄糖和MPV值有显着差异，该差异在储存第5天得到解决。 T2 中的 PC 在整个储存过程中的 pH、葡萄糖和 MPV 水平存在显着差异。直到储存第 42 天，测试组和对照组的 PRBC 具有相似的质量参数。直到保存 3 个月，所有测试中的 FFP 均不劣于同期对照组。结论 过夜保存 WB 没有发生持久的有害变化。尽管测试组中的一些生化参数存在显着差异，但可以接受它们以改善组分分离的物流。总体而言，在隔夜持有后，PRP 方法似乎比 BC 方法有更好的结果。