In recent years, researchers have increasingly focused on the development of multiphase trimetallic nanocomposites (TMNC) incorporating ternary metals or metal oxides, which hold significant potential as alternatives for combatting biofilms and bacterial infections. Enhanced oral health is ensured by the innovative techniques used to effectively prevent bacterial adherence and formation of biofilm on dental sutures. In this investigation, TMNC, which consists of Pb, Ag, and Cu, was synthesized using an autoclave-assisted sonochemical technique. Following synthesis, TMNC were characterized using FTIR, XRD, BET, XPS, TGA, and Raman spectroscopy to analyze their shape and microstructure. Subsequent evaluations, including MTT assay, antibacterial activity testing, and biofilm formation analysis, were conducted to assess the efficiency of the synthesized TMNC. Cytotoxicity and anti-human oral squamous cell carcinoma activities of TMNC were evaluated using the Human Oral Cancer cell line (KB) cell line through MTT assay, demonstrating a dose-dependent increase in anti-human oral squamous cell carcinoma activity against the KB cell line compared to the normal cell line, resulting in notably high cell viability. Furthermore, an ultrasonic probe was employed to incorporate TMNC onto dental suturing threads, with different concentrations of TMNC, ultrasonic power levels, and durations considered to determine optimal embedding conditions that result in the highest antibacterial activity. The inhibitory effects of TMNC, both in well diffusion assays and when incorporated into dental suturing threads, against gram-positive () and gram-negative () bacteria on Mueller-Hinton agar (MHA) were assessed using various concentrations of TMNC. The results of the study indicated that the efficacy of TMNC in inhibiting bacterial growth on dental suturing threads remained impressive, even at low concentrations. Moreover, an evaluation of their potential to destabilize biofilms formed by and , the two pathogens in humans, indicated that TMNC would be a promising anti-biofilm agent.

中文翻译：

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通过高压釜辅助声化学合成 Pb/Ag/Cu 三金属纳米复合材料增强在牙科中的应用


近年来，研究人员越来越关注包含三元金属或金属氧化物的多相三金属纳米复合材料（TMNC）的开发，它们作为对抗生物膜和细菌感染的替代品具有巨大的潜力。创新技术可有效防止牙齿缝线上的细菌粘附和生物膜形成，从而确保增强口腔健康。在这项研究中，TMNC 由 Pb、Ag 和 Cu 组成，是使用高压釜辅助声化学技术合成的。合成后，使用 FTIR、XRD、BET、XPS、TGA 和拉曼光谱对 TMNC 进行表征，以分析其形状和微观结构。随后进行了MTT测定、抗菌活性测试和生物膜形成分析等评估，以评估合成的TMNC的效率。使用人口腔癌细胞系（KB）细胞系通过 MTT 测定评估 TMNC 的细胞毒性和抗人口腔鳞状细胞癌活性，证明对 KB 细胞系的抗人口腔鳞状细胞癌活性呈剂量依赖性增加与正常细胞系相比，细胞活力显着提高。此外，采用超声波探头将 TMNC 整合到牙科缝合线上，考虑不同的 TMNC 浓度、超声波功率水平和持续时间，以确定产生最高抗菌活性的最佳嵌入条件。使用不同浓度的 TMNC 评估了 TMNC 在良好扩散测定中和掺入牙科缝合线时对 Mueller-Hinton 琼脂 (MHA) 上革兰氏阳性 () 和革兰氏阴性 () 细菌的抑制作用。 研究结果表明，即使在低浓度下，TMNC 在抑制牙科缝合线细菌生长方面的功效仍然令人印象深刻。此外，对它们破坏人类两种病原体形成的生物膜的潜力的评估表明，TMNC 将是一种有前途的抗生物膜剂。