In the current study, a novel crude polysaccharide (cNCEP) was extracted from Vaucher utilizing ultrasonic-assisted extraction (UAE) with 60 % ethanol, employing response surface methodology. The optimal yield of cNCEP was determined to be 8.07 ± 0.08 mg/g, achieved through ultrasonic-assisted extraction under the conditions of a material-to-liquid ratio of 1:22, temperature of 56 °C, power of 570 W, and duration of 147 min. Subsequent purification of NCEP via Sephadex G75 resulted in a novel polysaccharide with a molecular weight of 20.466 kDa. NCEP exhibited significant scavenging activites against DPPH and hydroxyl radicals, as well as notable immunomodulatory properties. Furthermore, the mechanisms underlying the immunomodulatory effects of NCEP, involving enhancement of immunity, were investigated, revealing potential regulation of MAPK and TLR4-IRF7-NF-κB signaling pathways through RNA-Seq and Western blot analyses. These findings highlight the promising potential of NCEP as an organic immunomodulatory agent and functional food ingredient.

中文翻译：

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超声辅助提取发菜低分子量多糖及其结构表征和免疫调节活性


在当前的研究中，采用响应面法，采用超声波辅助提取 (UAE) 和 60% 乙醇，从 Vaucher 中提取了一种新型粗多糖 (cNCEP)。在料液比1:22、温度56℃、功率570W、持续时间 147 分钟。随后通过 Sephadex G75 纯化 NCEP，得到分子量为 20.466 kDa 的新型多糖。 NCEP 对 DPPH 和羟基自由基具有显着的清除活性，并且具有显着的免疫调节特性。此外，还研究了 NCEP 免疫调节作用（涉及增强免疫力）的潜在机制，通过 RNA-Seq 和蛋白质印迹分析揭示了 MAPK 和 TLR4-IRF7-NF-κB 信号通路的潜在调节作用。这些发现凸显了 NCEP 作为有机免疫调节剂和功能性食品成分的巨大潜力。