Biothiol assays offer vital insights into health assessment and facilitate the early detection of potential health issues, thereby enabling timely and effective interventions. In this study, we developed ultrasmall CuMn-Histidine (His) nanozymes with multiple enzymatic activities. CuMn-His enhanced peroxidase (POD)-like activity at neutral pH was achieved through hydrogen bonding and electrostatic effects. In addition, CuMn-His possesses laccase (LAC)-like and superoxide dismutase (SOD)-like activities at neutral pH. Based on three different enzyme mimetic activities of CuMn-His at neutral pH, the colorimetric sensing array without changing the buffer solution was successfully constructed. The array was successfully used for the identification of three biothiols, glutathione (GSH), cysteine (Cys), and homocysteine (Hcy). Subsequently, excellent application results were shown in complex serum and cellular level analyses. This study provides an innovative strategy for the development of ultrasmall bimetallic nanozymes with multiple enzymatic activities and the construction of colorimetric sensing arrays.

中文翻译：

---

中性 pH 下具有多酶活性的超小型 CuMn-His 纳米酶：构建用于生物硫醇检测和疾病识别的比色传感阵列


生物硫醇测定为健康评估提供了重要的见解，并有助于及早发现潜在的健康问题，从而实现及时有效的干预。在这项研究中，我们开发了具有多种酶活性的超小型 CuMn-组氨酸 (His) 纳米酶。 CuMn-His 通过氢键和静电效应增强了中性 pH 下的过氧化物酶 (POD) 样活性。此外，CuMn-His 在中性 pH 下具有漆酶 (LAC) 样和超氧化物歧化酶 (SOD) 样活性。基于CuMn-His在中性pH下的三种不同的酶模拟活性，成功构建了不改变缓冲溶液的比色传感阵列。该芯片成功用于鉴定三种生物硫醇：谷胱甘肽 (GSH)、半胱氨酸 (Cys) 和同型半胱氨酸 (Hcy)。随后，在复杂的血清和细胞水平分析中显示出优异的应用结果。这项研究为开发具有多种酶活性的超小型双金属纳米酶和构建比色传感阵列提供了创新策略。