Protein coronas provide the biological identity of nanomaterials in vivo. Here we have used dynamic light scattering (DLS) and transmission electron microscopy (TEM) to investigate the adsorption of serum proteins, including bovine serum albumin (BSA), transferrin (TRF) and fibrinogen (FIB), on gold nanoparticles (AuNPs) with different surface modifications (citrate, thioglycolic acid, cysteine, polyethylene glycol (PEG, M _w = 2 k and 5 k)). AuNPs with PEG(5 k) surface modification showed no protein adsorption. AuNPs with non-PEG surface modifications showed aggregation with FIB. AuNPs with citrate and thioglycolic acid surface modifications showed 6–8 nm thick BSA and TRF coronas (corresponding to monolayer or bilayer proteins), in which the microscopic dissociation constants of BSA and TRF protein coronas are in the range of 10⁻⁸ to 10⁻⁶ M.

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中文翻译：

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具有不同表面修饰的金纳米粒子上蛋白质电晕的定量研究

蛋白质电晕提供了体内纳米材料的生物学特性。在这里，我们使用动态光散射（DLS）和透射电子显微镜（TEM）来研究血清蛋白，包括牛血清白蛋白（BSA），转铁蛋白（TRF）和纤维蛋白原（FIB）在金纳米颗粒（AuNPs）上的吸附。不同的表面修饰（柠檬酸盐，巯基乙酸，半胱氨酸，聚乙二醇（PEG，M _w= 2 k和5 k））。具有PEG（5 k）表面修饰的AuNPs没有蛋白质吸附。具有非PEG表面修饰的AuNPs与FIB聚集在一起。用柠檬酸盐和巯基乙酸的表面修饰的AuNP表明6-8纳米厚的BSA和TRF电晕（对应于单层或双层的蛋白质），其中BSA和TRF蛋白电晕的微观解离常数是在10〜^-8〜10 ^{- 6} M.

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