In this study, a new diagnostic assay to detect , the causative agent of mal secco of citrus, was developed based on the recombinase polymerase amplification (RPA) technology. Mal secco is a well-known and damaging vascular disease, affecting primarily lemon () and, to a lesser extent, other citrus species, including those in the genera and . The disease poses a considerable threat to lemon production in most of the citrus-producing countries of the Mediterranean region and in the Black Sea area. RPA primers and probes were designed to amplify a 142 bp amplicon from the ITS regions of . The inclusivity and specificity of the RPA assay were tested on gDNA isolated from a panel including 29 strains of various origin of and 18 non-target fungal and oomycete plant pathogens typically isolated from citrus trees. The assay was specific to and had a detection threshold of 1.0 pg of gDNA. Preliminary tests carried out on plant crude extract highlighted RPA's potential for the rapid, user-friendly, and cost-effective field diagnosis of mal secco.

中文翻译：

---

一种基于荧光的便携式重组酶聚合酶扩增测定法，用于检测 Plenodomus tracheiphilus 的 Mal secco 病


在这项研究中，基于重组酶聚合酶扩增（RPA）技术开发了一种新的诊断方法来检测柑橘霉病的病原体。 Mal secco 是一种众所周知的破坏性血管疾病，主要影响柠檬，并在较小程度上影响其他柑橘品种，包括柑橘属和柑橘属的柑橘属。该疾病对地中海地区和黑海地区大多数柑橘生产国的柠檬生产构成了相当大的威胁。 RPA 引物和探针设计用于从 的 ITS 区域扩增 142 bp 扩增子。 RPA 测定的包容性和特异性在从一组分离的 gDNA 上进行测试，该组包括 29 种不同来源的菌株和 18 种通常从柑橘树中分离的非目标真菌和卵菌植物病原体。该检测针对 gDNA，检测阈值为 1.0 pg。对植物粗提物进行的初步测试突显了 RPA 在快速、用户友好且经济高效的 Mal secco 现场诊断方面的潜力。