Stimulating the biofilm formation of Bacillus populations to mitigate soil antibiotic resistome during insect fertilizer application,Environment International

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Stimulating the biofilm formation of Bacillus populations to mitigate soil antibiotic resistome during insect fertilizer application
Environment International ( IF 10.3 ) Pub Date : 2024-06-18 , DOI: 10.1016/j.envint.2024.108831
Zhengzheng Zhao ₁ , Bingqi Gao ₁ , Chongrui Yang ₁ , Yushi Wu ₁ , Chen Sun ₁ , Núria Jiménez ₂ , Longyu Zheng ₁ , Feng Huang ₁ , Zhuqing Ren ₃ , Ziniu Yu ₁ , Chan Yu ₄ , Jibin Zhang ₁ , Minmin Cai ₁

Affiliation

Antibiotic resistance in soil introduced by organic fertilizer application pose a globally recognized threat to human health. Insect organic fertilizer may be a promising alternative due to its low antibiotic resistance. However, it is not yet clear how to regulate soil microbes to reduce antibiotic resistance in organic fertilizer agricultural application. In this study, we investigated soil microbes and antibiotic resistome under black soldier fly organic fertilizer (BOF) application in pot and field systems. Our study shows that BOF could stimulate ARB (antibiotic resistant − bacteria) − suppressive in the soil microbiome and reduce antibiotic resistome. The carbohydrate transport and metabolism pathway of soil was strengthened, which accelerated the synthesis and transport of polysaccharides to form biofilm to antagonistic soil ARB, and thus reduced the antibiotic resistance. We further tested the ARB − suppressive spp. in a microcosm assay, which resulted in a significant decrease in the presence of ARGs and ARB together with higher abundance in key biofilm formation gene (). This knowledge might help to the development of more efficient bio-fertilizers aimed at mitigating soil antibiotic resistance and enhancing soil health, in particular, under the requirements of global “One Health”.

中文翻译：

刺激芽孢杆菌种群生物膜形成以减轻昆虫肥料施用期间土壤抗生素抗性

有机肥施用引起的土壤抗生素耐药性对人类健康构成了全球公认的威胁。由于其抗生素抗性较低，昆虫有机肥可能是一种有前途的替代品。然而，在有机肥农业施用中如何调节土壤微生物以减少抗生素耐药性尚不清楚。在这项研究中，我们研究了盆栽和田间系统施用黑水虻有机肥（BOF）下的土壤微生物和抗生素抗性组。我们的研究表明，BOF 可以刺激土壤微生物组中的 ARB（抗生素耐药性细菌）抑制并减少抗生素耐药性。土壤碳水化合物运输和代谢途径得到加强，加速了多糖的合成和运输，形成生物膜对抗土壤ARB，从而降低抗生素耐药性。我们进一步测试了 ARB - 抑制性物种。在微观分析中，这导致 ARG 和 ARB 的存在显着减少，同时关键生物膜形成基因的丰度增加 ()。这些知识可能有助于开发更有效的生物肥料，旨在减轻土壤抗生素耐药性和增强土壤健康，特别是在全球“同一个健康”的要求下。

更新日期：2024-06-18

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