Bone defects caused by trauma, fracture-related infection, congenital malformations, and tumor resection remain significant challenges to human health. Biodegradable Zn-based alloys have been acknowledged for their outstanding comprehensive properties in addressing bone defects, offering adequate mechanical support, mild degradation rate, and satisfactory biosafety. In this study, Zn–0.8Li-0.1Ca alloy was fabricated and comprehensively evaluated both and for their properties. The Zn-0.8Li-0.1Ca alloy demonstrated an ultimate tensile strength of 251.07 ± 9.59 MPa and an elongation of 18.89 ± 1.26 %. Electrochemical and degradation tests showed that there was a mild degradation rate (21.94 ± 2.26 μm/year) and uniform degradation behavior in the Zn-0.8Li-0.1Ca alloy. Real-time PCR analysis revealed that the Zn-0.8Li-0.1Ca alloy effectively upregulated the expression levels of osteogenesis-related genes (ALP, COL-1, OCN, and RUNX-2). Micro-CT and histological evaluations demonstrated that bone regeneration within the Zn-0.8Li-0.1Ca alloy scaffold was significantly superior to that of pure Ti in repairing rat femoral condyle defects. Therefore, the biodegradable Zn-0.8Li-0.1Ca ternary alloy displayed satisfactory mechanical properties, degradation behavior, biocompatibility, and osteogenic activity, positioning it as a promising candidate for bone repair treatment.

中文翻译：

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用于骨再生的可生物降解的 Zn-Li-Ca 三元合金的生物相容性和骨诱导性：体外和体内研究


外伤、骨折相关感染、先天畸形和肿瘤切除引起的骨缺损仍然对人类健康构成重大挑战。可生物降解的锌基合金以其在解决骨缺损、提供足够的机械支撑、温和的降解率和令人满意的生物安全性方面的出色综合性能而受到认可。在本研究中，制备了 Zn-0.8Li-0.1Ca 合金并对其性能进行了综合评估。 Zn-0.8Li-0.1Ca合金的极限拉伸强度为251.07±9.59MPa，伸长率为18.89±1.26%。电化学和降解测试表明Zn-0.8Li-0.1Ca合金具有温和的降解速率（21.94±2.26μm/年）和均匀的降解行为。实时PCR分析表明，Zn-0.8Li-0.1Ca合金有效上调成骨相关基因（ALP、COL-1、OCN和RUNX-2）的表达水平。显微CT和组织学评估表明，Zn-0.8Li-0.1Ca合金支架内的骨再生在修复大鼠股骨髁缺损方面明显优于纯钛支架。因此，可生物降解的Zn-0.8Li-0.1Ca三元合金表现出令人满意的机械性能、降解行为、生物相容性和成骨活性，使其成为骨修复治疗的有希望的候选者。