Metabolic burden is defined by the influence of genetic manipulation and environmental perturbations on the distribution of cellular resources. The rewiring of microbial metabolism for bio-based chemical production often leads to a metabolic burden, followed by adverse physiological effects, such as impaired cell growth and low product yields. Alleviating the burden imposed by undesirable metabolic changes has become an increasingly attractive approach for constructing robust microbial cell factories. In this review, we provide a brief overview of metabolic burden engineering, focusing specifically on recent developments and strategies for diminishing the burden while improving robustness and yield. A variety of examples are presented to showcase the promise of metabolic burden engineering in facilitating the design and construction of robust microbial cell factories. Finally, challenges and limitations encountered in metabolic burden engineering are discussed.

中文翻译：

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减轻代谢负担以提高工业微生物的稳健性和生物生产


代谢负担是由基因操纵和环境扰动对细胞资源分布的影响来定义的。生物基化学品生产中微生物代谢的重新布线通常会导致代谢负担，进而产生不利的生理效应，例如细胞生长受损和产品产量低。减轻不良代谢变化带来的负担已成为构建强大的微生物细胞工厂越来越有吸引力的方法。在这篇综述中，我们简要概述了代谢负担工程，特别关注最近的发展和减轻负担同时提高稳健性和产量的策略。提出了各种例子来展示代谢负荷工程在促进强大的微生物细胞工厂的设计和建造方面的前景。最后，讨论了代谢负荷工程中遇到的挑战和限制。