The properties of DNA that make it an effective genetic material also allow it to be ideal for programmed self-assembly. Such DNA-programmed assembly has been utilized to construct responsive DNA origami and wireframe nanoassemblies, yet replicating these hybrid nanomaterials remains challenging. Here we report a strategy for replicating DNA wireframe nanoassemblies using the isothermal ligase chain reaction lesion-induced DNA amplification (LIDA). We designed a triangle wireframe structure that can be formed in one step by ring-closing of its linear analog. Introducing a small amount of the wireframe triangle to an excess of the linear analog and complementary fragments, one of which contains a destabilizing abasic lesion, leads to rapid, sigmoidal self-replication of the wireframe triangle via cross-catalysis. Using the same cross-catalytic strategy we also demonstrate rapid self-replication of a hybrid wireframe triangle containing synthetic vertices as well as the self-replication of circular DNA. This work reveals the suitability of isothermal ligase chain reactions such as LIDA to self-replicate complex DNA architectures, opening the door to incorporating self-replication, a hallmark of life, into biomimetic DNA nanotechnology.

中文翻译：

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基于 DNA 的自我复制纳米组件


DNA 的特性使其成为一种有效的遗传物质，也使其成为程序化自组装的理想选择。这种 DNA 编程组装已被用来构建响应性 DNA 折纸和线框纳米组装，但复制这些混合纳米材料仍然具有挑战性。在这里，我们报告了一种使用等温连接酶链反应损伤诱导 DNA 扩增 (LIDA) 复制 DNA 线框纳米组件的策略。我们设计了一种三角形线框结构，可以通过其线性模拟的闭环一步形成。将少量的线框三角形引入过量的线性模拟和互补片段（其中之一包含不稳定的无碱基损伤）中，通过交叉催化导致线框三角形快速、S形自我复制。使用相同的交叉催化策略，我们还证明了包含合成顶点的混合线框三角形的快速自我复制以及环状 DNA 的自我复制。这项工作揭示了 LIDA 等等温连接酶链反应对自我复制复杂 DNA 结构的适用性，为将自我复制（生命的标志）融入仿生 DNA 纳米技术打开了大门。