The prohibition of processed animal proteins (PAPs) has been relaxed gradually since 2007. The official control method for PAPs in feedingstuff, a combination of light microscopy (LM) followed by PCR, is no longer sufficient. Thus, a targeted LC–MS/MS method was developed, which enables a tissue-specific distinction between egg and dairy products, gelatine, and PAPs derived from blood or muscle tissue of the species ruminants, pigs, poultry, and fish. Tissue-specific proteins were analyzed after tryptic digestion to peptides with high-resolution ESI-QTOF-MS. A targeted method was developed based on untargeted proteomics approaches and the selection of specific peptides (45 unique peptides in total). Proficiency testing of blank and spiked samples revealed excellent results for trueness and selectivity. Furthermore, sensitivity was achieved at a level of 0.1% (w/w) for assessed peptides. Summing up, the developed method seems to be suitable for routine analysis after verification by ring trials.

中文翻译：

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采用靶向高分辨率 LC-MS/MS 同时测定饲料中反刍动物、猪、家禽和鱼类的动物产品


自 2007 年以来，加工动物蛋白 (PAP) 的禁令逐渐放松。饲料中 PAP 的官方控制方法，即光学显微镜 (LM) 和 PCR 相结合，已不再足够。因此，开发了一种有针对性的 LC-MS/MS 方法，该方法能够对来自反刍动物、猪、家禽和鱼类的血液或肌肉组织的鸡蛋和乳制品、明胶和 PAP 进行组织特异性区分。胰蛋白酶消化肽后，使用高分辨率 ESI-QTOF-MS 分析组织特异性蛋白质。基于非靶向蛋白质组学方法和特定肽（总共 45 种独特肽）的选择，开发了一种靶向方法。空白和加标样品的能力验证显示出良好的真实性和选择性结果。此外，所评估的肽的灵敏度达到 0.1% (w/w)。综上所述，经环试验验证，该方法适合常规分析。