Egg white hydrolysates (EWH) and ovotransferrin-derived peptides have distinct beneficial effects on glucose metabolism. This research aims to investigate whether ovalbumin hydrolysates (OVAHs), without ovotransferrin can improve insulin signaling pathway in high-fat diet (HFD)-fed mice. Two types of ovalbumin hydrolysates were produced, either using thermoase (OVAT), or thermoase + pepsin (OVATP). Both OVAHs-supplemented groups exhibited lower body weight gain (P < 0.001) and enhanced oral glucose tolerance (P < 0.05) compared with HFD. Moreover, diet supplementation with either hydrolysate increased the insulin-stimulated activation of protein kinase B (AKT) and insulin receptor β (IRβ) (P < 0.0001) in skeletal muscle. In conclusion, OVAHs improved glucose tolerance and insulin-dependent signaling pathway in HFD-fed mice.

中文翻译：

---

卵清蛋白水解物增强高脂饮食喂养小鼠的骨骼肌胰岛素依赖性信号通路


蛋清水解物 (EWH) 和卵转铁蛋白衍生肽对葡萄糖代谢具有明显的有益作用。本研究旨在探讨不含卵转铁蛋白的卵清蛋白水解物 (OVAH) 是否可以改善高脂饮食 (HFD) 喂养小鼠的胰岛素信号通路。使用热酶 (OVAT) 或热酶 + 胃蛋白酶 (OVATP) 生产两种类型的卵清蛋白水解产物。与 HFD 相比，补充 OVAH 的两组均表现出较低的体重增加（P < 0.001）和增强的口服葡萄糖耐量（P < 0.05）。此外，饮食中添加任一水解产物都会增加骨骼肌中胰岛素刺激的蛋白激酶 B (AKT) 和胰岛素受体 β (IRβ) 的激活（P < 0.0001）。总之，OVAH 改善了 HFD 喂养小鼠的葡萄糖耐量和胰岛素依赖性信号通路。