Insecticide susceptibility is mainly determined by the insect host, but symbiotic bacteria are also an important affecting factor. In this study, we investigate the relationship between the structure of gut bacterial symbionts and insecticide susceptibility in Diaphorina citri, the important carrier of Candidatus Liberibacter asiaticus (CLas), the causal agent of Huanglongbing (HLB). Our results indicated that antibiotic treatment significantly increased the susceptibility of D. citri to bifenthrin and thiamethoxam, and significantly decreased the relative abundance of Wolbachia and Profftella, enzyme activities of CarEs, and expression level of multiple CarE genes. The relative loads of Wolbachia and Profftella were positively correlated with DcitCCE13, DcitCCE14, DcitCCE15, and DcitCCE16. RNAi and prokaryotic expression revealed that DcitCCE15 is associated with bifenthrin metabolism. These results revealed that bacterial symbionts might regulate DcitCCE15 expression, which is involved in the susceptibility of D. citri to bifenthrin.

中文翻译：

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细菌共生体通过改变宿主解毒基因的表达水平导致柑橘木甲的杀虫剂敏感性


杀虫剂的敏感性主要由昆虫宿主决定，但共生菌也是重要的影响因素。在本研究中，我们研究了柑橘木虱肠道细菌共生体的结构与杀虫剂敏感性之间的关系，柑橘木虱是黄龙病（HLB）致病菌亚洲自由杆菌（CLas）的重要载体。我们的结果表明，抗生素处理显着增加了柑橘果蝇对联苯菊酯和噻虫嗪的敏感性，并显着降低了沃尔巴克氏菌和普罗夫特氏菌的相对丰度、CarEs的酶活性以及多个CarE基因的表达水平。 Wolbachia 和 Profftella 的相对负载量与 DcitCCE13、DcitCCE14、DcitCCE15 和 DcitCCE16 呈正相关。 RNAi 和原核表达表明 DcitCCE15 与联苯菊酯代谢相关。这些结果表明，细菌共生体可能调节 DcitCCE15 的表达，该表达与柑橘皮蝇对联苯菊酯的敏感性有关。