A new fusicoccane diterpenoid, harziaderma A (1), two novel harziane diterpenoids, harzianones G and H (2 and 3), one revised harziane diterpenoid (4), and two known diterpenoids (5 and 6) were isolated from the fungus Trichoderma harzianum and established via NMR, HRESIMS, Mo₂(OAc)₄-induced circular dichroism (ICD) and electronic circular dichroism (ECD) calculations. It is worth noting that compound 1 represents the first instance of a fusicoccane-type diterpenoid derived from T. harzianum. The structure of furanharzianone B was revised to 4 via careful spectroscopic analyses. Additionally, compounds 2 and 5 could suppress the overall growth of the foodborne bacterial pathogen Bacillus cereus. Compound 4 showed a moderate suppressive impact on NO generation in lipopolysaccharide (LPS)-treated RAW 264.7 cells. The discoveries from the current study not only expanded the structural variety of diterpenoids isolated from T. harzianum but also laid a robust foundation for the development of harziane diterpenoids as anti-foodborne pathogen agents.

中文翻译：

---

哈茨木霉中具有抗菌活性的二萜类化合物


从真菌哈茨木霉 (Trichoderma harzianum) 中分离出一种新的 fusicoccane 二萜类化合物 harziaderma A (1)、两种新型的 harziane 二萜类化合物 harzianenesone G 和 H（2 和 3）、一种改良的 harziane 二萜类化合物 (4) 和两种已知的二萜类化合物（5 和 6）并通过NMR、HRESIMS、Mo ₂ (OAc) ₄ 诱导圆二色性(ICD) 和电子圆二色性(ECD) 计算确定。值得注意的是，化合物 1 代表了源自 T. harzianum 的 fusicoccane 型二萜类化合物的第一个实例。通过仔细的光谱分析，呋喃哈齐酮 B 的结构被修改为 4。此外，化合物 2 和 5 可以抑制食源性细菌病原体蜡样芽孢杆菌的整体生长。化合物 4 对脂多糖 (LPS) 处理的 RAW 264.7 细胞中的 NO 产生具有中等程度的抑制作用。本研究的发现不仅扩大了从哈茨木霉中分离的二萜类化合物的结构多样性，而且为哈茨烷二萜类化合物作为抗食源性病原体的开发奠定了坚实的基础。