Antibiotic residues may be present in fruit products from trees that were treated to combat bacterial diseases such as citrus greening or blight. A liquid chromatography–high-resolution mass spectrometry (LC–HRMS) method was developed for the simultaneous determination and identification of streptomycin, kasugamycin, penicillin, and oxytetracycline residues in fruit. Samples were extracted with acidic methanol and separation was optimized for a hydrophilic interaction LC column. A Q-Exactive HRMS instrument was used to obtain product ion spectra for analyte identification. Quantitation was performed with matrix-extracted calibration curves and internal standard correction. The method was tested on many different types of fruit. In general, fortified samples demonstrated acceptable recoveries (82–116%) and reproducibility (<15% RSD). Method detection limits for these analytes were well below the established US EPA tolerance levels. It was also possible to analyze the fruit extracts prepared using this method for additional chemical contaminants using LC-HRMS.

中文翻译：

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使用液相色谱-高分辨率质谱 (LC-HRMS) 测定和鉴定水果中的抗生素残留


经过处理的树木的水果产品中可能存在抗生素残留，这些水果产品经过处理以对抗细菌性疾病，例如柑橘黄龙病或枯萎病。建立了同时测定和鉴定水果中链霉素、春雷霉素、青霉素和土霉素残留量的液相色谱-高分辨质谱（LC-HRMS）方法。用酸性甲醇萃取样品，并针对亲水相互作用液相色谱柱优化分离。使用 Q-Exactive HRMS 仪器获得产物离子谱以进行分析物鉴定。使用基质提取的校准曲线和内标校正进行定量。该方法在许多不同类型的水果上进行了测试。一般来说，强化样品表现出可接受的回收率 (82-116%) 和重现性 (<15% RSD)。这些分析物的方法检测限远低于美国 EPA 规定的耐受水平。还可以使用 LC-HRMS 分析使用此方法制备的水果提取物中的其他化学污染物。