Probiotics can regulate gut microbiota and protect against acute alcohol-induced liver injury through the gut–liver axis. However, efficacy is strain-dependent, and their mechanism remains unclear. This study investigated the effect of lactic acid bacteria (LAB), including Lacticaseibacillus paracasei E10 (E10), Lactiplantibacillus plantarum M (M), Lacticaseibacillus rhamnosus LGG (LGG), Lacticaseibacillus paracasei JN-1 (JN-1), and Lacticaseibacillus paracasei JN-8 (JN-8), on the prevention of acute alcoholic liver injury in mice. We found that LAB pretreatment reduced serum alanine transaminase (ALT) and aspartate transaminase (AST) and reduced hepatic total cholesterol (TC) and triglyceride (TG). JN-8 pretreatment exhibited superior efficacy in improving hepatic antioxidation. LGG and JN-8 pretreatment significantly attenuated hepatic and colonic inflammation by decreasing the expression of interleukin 6 (IL-6) and tumor necrosis factor α (TNF-α) and increasing the expression of interleukin 10 (IL-10). JN-1 and JN-8 pretreatments have better preventive effects than other LAB pretreatment on intestinal barrier dysfunction. In addition, the LAB pretreatment improved gut microbial dysbiosis and bile acid (BA) metabolic abnormality. All of the strains were confirmed to have bile salt deconjugation capacities in vitro, where M and JN-8 displayed higher activities. This study provides new insights into the prevention and mechanism of LAB strains in preventing acute alcoholic liver injury.

中文翻译：

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乳酸菌通过肠道微生物群-胆汁酸-肝轴预防急性酒精性肝损伤的能力差异


益生菌可以调节肠道微生物群，并通过肠-肝轴防止急性酒精引起的肝损伤。然而，功效取决于菌株，其机制仍不清楚。本研究调查了乳酸菌 (LAB) 的作用，包括副干酪乳杆菌 E10 (E10)、植物乳杆菌 M (M)、鼠李糖乳杆菌 LGG (LGG)、副干酪乳杆菌 JN-1 (JN-1) 和副干酪乳杆菌 JN -8（JN-8），对小鼠急性酒精性肝损伤的预防作用。我们发现 LAB 预处理降低了血清丙氨酸转氨酶 (ALT) 和天冬氨酸转氨酶 (AST)，并降低了肝脏总胆固醇 (TC) 和甘油三酯 (TG)。 JN-8预处理在改善肝脏抗氧化方面表现出优异的功效。 LGG 和 JN-8 预处理通过降低白细胞介素 6 (IL-6) 和肿瘤坏死因子 α (TNF-α) 的表达并增加白细胞介素 10 (IL-10) 的表达，显着减轻肝脏和结肠炎症。 JN-1和JN-8预处理对肠道屏障功能障碍的预防效果优于其他LAB预处理。此外，LAB预处理改善了肠道微生物失调和胆汁酸（BA）代谢异常。所有菌株均被证实具有体外解胆盐能力，其中M和JN-8表现出更高的活性。该研究为LAB菌株预防急性酒精性肝损伤的预防和机制提供了新的见解。