Resveratrol, a natural polyphenol compound used as an ingredient in dietary supplements, and pharmaceuticals, has gained significant attention due to its potential health benefits. However, the accurate and sensitive determination of resveratrol in complex matrices remains a challenge. In this study, we propose the utilization of bimetallic porous Mn/Co oxide nanosheets (MnCoO-NSs) as catalysts for the colorimetric determination of resveratrol. The bimetallic porous MnCoO-NSs were prepared through a facile one-stone-two-birds strategy. These nanosheets exhibited superior oxidase-mimicking activity, as evidenced by the catalytic oxidation of the chromogenic substrate 3,3′,5,5′-tetramethylbenzidine (TMB), producing a blue-colored oxTMB species with a prominent absorbance peak at 655 nm. The catalytic activity was promoted through the production of superoxide anion (O), which enhanced the affinity of MnCoO-NSs to the TMB molecules. Upon the addition of resveratrol, the oxidation process was inhibited, resulting in rapid fading of the blue color. This colorimetric sensing platform exhibited a linear response to resveratrol concentrations over the range of 2.2–87.6 μM, with a limit of detection of 0.210 μM. The method was further applied for the determination of resveratrol in different matrices including biological fluids, pharmaceuticals, and environmental water. The utilization of these MnCoO-NSs offers a simple and cost-effective alternative to conventional analytical techniques for the determination of resveratrol. Their high sensitivity, selectivity, and stability enable accurate measurements of resveratrol in various complex matrices. This research has implications in areas such as pharmaceutical analysis, biomedical research, and environmental analysis, where the reliable determination of resveratrol is crucial for assessing its therapeutic potential and ensuring product quality.

中文翻译：

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具有高效氧化酶模拟活性的双金属多孔锰/钴氧化物纳米片，用于不同基质中白藜芦醇的灵敏比色测定


白藜芦醇是一种天然多酚化合物，用作膳食补充剂和药品的成分，由于其潜在的健康益处而受到广泛关注。然而，准确、灵敏地测定复杂基质中的白藜芦醇仍然是一个挑战。在这项研究中，我们建议利用双金属多孔锰/钴氧化物纳米片（MnCoO-NSs）作为比色测定白藜芦醇的催化剂。双金属多孔 MnCoO-NSs 是通过简单的一石二鸟策略制备的。这些纳米片表现出优异的氧化酶模拟活性，显色底物 3,3',5,5'-四甲基联苯胺 (TMB) 的催化氧化证明了这一点，产生了在 655 nm 处具有突出吸光度峰的蓝色 oxTMB 物质。通过产生超氧阴离子（O）来提高催化活性，从而增强了 MnCoO-NSs 与 TMB 分子的亲和力。添加白藜芦醇后，氧化过程被抑制，导致蓝色快速褪色。该比色传感平台对白藜芦醇浓度在 2.2–87.6 μM 范围内表现出线性响应，检测限为 0.210 μM。该方法进一步应用于生物体液、药物和环境水等不同基质中白藜芦醇的测定。使用这些 MnCoO-NSs 为白藜芦醇的测定提供了一种简单且经济高效的替代传统分析技术的方法。它们的高灵敏度、选择性和稳定性能够准确测量各种复杂基质中的白藜芦醇。 这项研究对药物分析、生物医学研究和环境分析等领域具有重要意义，其中白藜芦醇的可靠测定对于评估其治疗潜力和确保产品质量至关重要。