Given the importance of achieving optimal therapeutical concentration in patients treated with antidepressants, this study investigates a novel technique for the simultaneous determination of trazodone (TRZ) and doxepin (DOX) in human plasma and serum samples for the first time. To achieve simultaneous determination of two antidepressants, TRZ and DOX, a novel detection system was designed: a non-enzymatic voltammetric biosensor based on boron-reduced graphene oxide/manganese oxide nanoparticles (GCE/B-rGO/MnO NPs). The detection was accomplished after pre-concentration and extraction trace amounts of the analytes using the thin film-solid phase microextraction (TF-SPME) technique, which employed polyvinyl alcohol/polyvinyl acetate/copper oxide nanoparticles (PVA/PVAc/CuO NPs) electrospun nanofibers. PVAc acts as a cross-linking agent for PVA, and their mixture is effective for sample preparation and pre-concentration of analytes in complex matrices. Also, adding CuO NPs to this polymeric mixture enhanced the adsorption efficiency.

中文翻译：

---

用于快速测定复杂样品中基于吸附剂的提取曲唑酮和多塞平的非酶电化学生物传感器的开发和表征


考虑到接受抗抑郁药物治疗的患者达到最佳治疗浓度的重要性，本研究首次研究了一种同时测定人血浆和血清样品中曲唑酮 (TRZ) 和多虑平 (DOX) 的新技术。为了实现TRZ和DOX两种抗抑郁药的同时测定，设计了一种新型检测系统：基于硼还原氧化石墨烯/氧化锰纳米粒子（GCE/B-rGO/MnO NPs）的非酶伏安生物传感器。使用薄膜固相微萃取 (TF-SPME) 技术预浓缩和提取痕量分析物后完成检测，该技术采用聚乙烯醇/聚醋酸乙烯酯/氧化铜纳米颗粒 (PVA/PVAc/CuO NPs) 电纺丝纳米纤维。 PVAc 作为 PVA 的交联剂，它们的混合物可有效用于样品制备和复杂基质中分析物的预浓缩。此外，向该聚合物混合物中添加 CuO NP 还提高了吸附效率。