Increasing evidence shows that microbial synthesis plays an important role in producing high value-added products. However, microbial monoculture generally hampers metabolites production and limits scalability due to the increased metabolic burden on the host strain. In contrast, co-culture is a more flexible approach to improve the environmental adaptability and reduce the overall metabolic burden. The well-defined co-culturing microbial consortia can tap their metabolic potential to obtain yet-to-be discovered and pre-existing metabolites. This review focuses on the use of a co-culture strategy and its underlying mechanisms to enhance the production of products. Notably, the significance of comprehending the microbial interactions, diverse communication modes, genetic information, and modular co-culture involved in co-culture systems were highlighted. Furthermore, it addresses the current challenges and outlines potential future directions for microbial co-culture. This review provides better understanding the diversity and complexity of the interesting interaction and communication to advance the development of co-culture techniques.

中文翻译：

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提高微生物共培养系统中代谢物产量的策略


越来越多的证据表明微生物合成在生产高附加值产品中发挥着重要作用。然而，由于宿主菌株的代谢负担增加，微生物单一培养通常会阻碍代谢物的产生并限制可扩展性。相比之下，共培养是一种更灵活的方法，可以提高环境适应性并减轻总体代谢负担。明确的共培养微生物群落可以挖掘其代谢潜力，以获得尚未发现和预先存在的代谢物。本综述的重点是使用共培养策略及其基本机制来提高产品的生产。值得注意的是，强调了理解共培养系统中涉及的微生物相互作用、不同的通讯模式、遗传信息和模块化共培养的重要性。此外，它还解决了当前的挑战，并概述了微生物共培养的潜在未来方向。本综述提供了更好地理解有趣的互动和交流的多样性和复杂性，以促进共培养技术的发展。