In pursuit of sustainable agricultural production, the development of environmentally friendly and effective biopesticides is essential to improve food security and environmental sustainability. Bacteriophages, as emerging biocontrol agents, offer an alternative to conventional antibiotics and synthetic chemical pesticides. The primary challenges in applying phage‐based biopesticides in agricultural settings are their inherent fragility and low biocidal efficacy, particularly the susceptibility to sunlight exposure. This study addresses the aforementioned challenges by innovatively encapsulating phages in sporopollenin exine capsules (SECs), which are derived from plant pollen grains. The size of the apertures on SECs could be controlled through a non‐thermal and rapid process, combining reinflation and vacuum infusion techniques. This unique feature facilitates the high‐efficiency encapsulation and controlled release of phages under various conditions. The proposed SECs could encapsulate over 9 log PFU g−1 of phages and significantly enhance the ultraviolet (UV) resistance of phages, thereby ensuring their enhanced survivability and antimicrobial efficacy. The effectiveness of SECs encapsulated phages (T7@SECs) in preventing and treating bacterial contamination on lettuce leaves is further demonstrated, highlighting the practical applicability of this novel biopesticide in field applications. Overall, this study exploits the potential of SECs in the development of phage‐based biopesticides, presenting a promising strategy to enhancing agricultural sustainability.

中文翻译：

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一种生态友好的自然启发微载体，用于增强噬菌体生物农药的递送、稳定性和杀菌功效


为了追求可持续农业生产，开发环境友好且有效的生物农药对于改善粮食安全和环境可持续性至关重要。噬菌体作为新兴的生物防治剂，提供了传统抗生素和合成化学农药的替代品。在农业环境中应用基于噬菌体的生物农药的主要挑战是其固有的脆弱性和低杀菌功效，特别是对阳光照射的敏感性。这项研究通过创新地将噬菌体封装在源自植物花粉粒的孢粉外壁胶囊（SEC）中来解决上述挑战。 SEC 上孔径的大小可以通过结合再充气和真空灌注技术的非热快速过程来控制。这种独特的功能有利于噬菌体在各种条件下的高效封装和受控释放。所提出的 SEC 可以封装超过 9 log PFU g−1 的噬菌体，并显着增强噬菌体的紫外线 (UV) 抵抗力，从而确保其增强的生存能力和抗菌功效。 SECs封装噬菌体（T7@SECs）在预防和处理生菜叶片细菌污染方面的有效性得到进一步证明，突出了这种新型生物农药在现场应用中的实际适用性。总体而言，这项研究利用了 SEC 在开发基于噬菌体的生物农药方面的潜力，为增强农业可持续性提出了一种有前景的策略。