For every epidemic outbreak, the prevention and treatments in resource‐limited areas are always out of reach. Critical to this is that high accuracy, stability, and more comprehensive analytical techniques always rely on expensive and bulky instruments and large laboratories. Here, a fully integrated and high‐throughput microfluidic system is proposed for ultra‐multiple point‐of‐care immunoassay, termed Dac system. Specifically, the Dac system only requires a handheld portable device to automatically recycle repetitive multi‐step reactions including on‐demand liquid releasing, dispensing, metering, collecting, oscillatory mixing, and discharging. The Dac system performs high‐precision enzyme‐linked immunosorbent assays for up to 17 samples or targets simultaneously on a single chip. Furthermore, reagent consumption is only 2% compared to conventional ELISA, and microbubble‐accelerated reactions shorten the assay time by more than half. As a proof of concept, the multiplexed detections are achieved by detecting at least four infection targets for two samples simultaneously on a singular chip. Furthermore, the barcode‐based multi‐target results can rapidly distinguish between five similar cases, allowing for accurate therapeutic interventions. Compared to bulky clinical instruments, the accuracy of clinical inflammation classification is 92.38% (n = 105), with a quantitative correlation coefficient of R2 = 0.9838, while the clinical specificity is 100% and the sensitivity is 98.93%.

中文翻译：

---

用于多重护理点测试的完全集成和高通量微流体系统


每一次疫情爆发，资源有限地区的防治总是遥不可及。对此至关重要的是，高精度、稳定性和更全面的分析技术始终依赖于昂贵且笨重的仪器和大型实验室。在此，提出了一种用于超多点护理免疫测定的完全集成的高通量微流体系统，称为 Dac 系统。具体来说，Dac系统只需要手持便携式设备即可自动回收重复的多步反应，包括按需液体释放、分配、计量、收集、振荡混合和排放。 Dac 系统可在单个芯片上同时对多达 17 个样品或靶标进行高精度酶联免疫吸附测定。此外，与传统 ELISA 相比，试剂消耗仅为 2%，并且微泡加速反应将检测时间缩短一半以上。作为概念证明，多重检测是通过在单个芯片上同时检测两个样本的至少四个感染目标来实现的。此外，基于条形码的多目标结果可以快速区分五个相似的病例，从而实现准确的治疗干预。与笨重的临床仪器相比，临床炎症分类的准确率为92.38%（n=105），定量相关系数R2=0.9838，而临床特异性为100%，敏感性为98.93%。