Nanoparticle-mediated photoporation has arisen as a universal intracellular delivery tool; however, the direct interaction of nanoparticles and cells hampers its clinical translation. Here, we report a uniform contactless intracellular delivery that transfects a large number of cells within a minute and avoids direct contact of nanoparticles and cells, thereby improving the cell viability. Our platform consists of an array of polydimethylsiloxane (PDMS) mixed reduced graphene oxide (rGO) nanoflakes on pyramidal microtips, uniformly distributed at the apex of the tip. The extraordinary optoelectronic properties of rGO were combined with micro-pyramidal cavities to entrap light in micro-cavities and efficiently convert it into heat through multiple reflections and absorptions. As a result, ultralow infra-red laser pulse irradiation could create cavitation bubbles followed by cell membrane deformation and biomolecular delivery. Using this delivery platform, we have achieved the delivery of small to large cargo (668 Da to 465 kDa) in various mammalian cells, including hard-to-transfect H9C2 cardiomyocytes. The best results were achieved for enzyme (465 kDa) delivery with a transfection efficiency and cell viability of 95% and 98%, respectively, in SiHa cells. The highly efficient cargo delivery tool demonstrated a safe and effective approach for cell therapy and diagnostics.

中文翻译：

---

使用 rGO 混合 PDMS 微尖装置将大量货物输送到难以转染的细胞中的超低强度光脉冲


纳米颗粒介导的光穿孔已成为一种通用的细胞内递送工具；然而，纳米粒子和细胞的直接相互作用阻碍了其临床转化。在这里，我们报告了一种均匀的非接触式细胞内递送，可在一分钟内转染大量细胞，并避免纳米粒子和细胞的直接接触，从而提高细胞活力。我们的平台由金字塔形微尖端上的聚二甲基硅氧烷（PDMS）混合还原氧化石墨烯（rGO）纳米片阵列组成，均匀分布在尖端的顶点。 rGO非凡的光电特性与微锥体空腔相结合，将光捕获在微空腔中，并通过多次反射和吸收有效地将其转化为热量。因此，超低红外激光脉冲照射可以产生空化气泡，随后导致细胞膜变形和生物分子传递。利用这个递送平台，我们已经实现了在各种哺乳动物细胞中递送小到大的货物（668 Da 到 465 kDa），包括难以转染的 H9C2 心肌细胞。在 SiHa 细胞中，酶 (465 kDa) 递送取得了最佳结果，转染效率和细胞活力分别为 95% 和 98%。这种高效的货物输送工具展示了一种安全有效的细胞治疗和诊断方法。