root is used in the polyherbal product ‘Dashmula’. Its exploitation for formulation preparation has depleted its availability, leading to medicine adulteration. Direct shoot regeneration from the cotyledonary node holds promise as a source of raw material. This study aimed to develop a regeneration protocol for and validate its genetic and metabolic fidelity. The seeds of showed low germination rates, prolonged dormancy, and poor viability. Root exploitation in the wild poses a threat to its availability in nature. Seedling’s derived cotyledonary nodes cultured on B5 medium supplemented with BAP (0.5–3 mg L), Kinetin (0.5–3 mg L), Thidiazuron (0.01–1 mg L), and -Topolin (0.1–4 mg L). To address hyperhydricity in regenerated shoots, cotyledonary nodes were cultured on high-agar concentration media. Microshoots were exposed to IBA solution (50–800 mg L) pulse treatment for rooting. Tissue-cultured plants genetic fidelity was assessed using ISSR and SCoT markers, while metabolic fidelity was studied with HRMS. The chlorophyll content, antioxidant, and antidiabetic activity of micropropagated plants were evaluated. The highest shoot regeneration frequency, with a maximum of 6.57±0.278 shoots per explant, was achieved using 2 mg LTopolin. The shoots were elongated, had expanded leaves, and were hyperhydrated. BAP (2 mg L) induced a maximum of 9.83±0.333 shoot buds per explant. BAP caused explant browning, profuse callus formation, dwarfing, and hyperhydric shoots. Hyperhydricity was alleviated with a higher agar concentration (1 %). IBA (400 mg L) induced a maximum of 2.18±0.090 roots per shoot and a root length of 9.23±0.033 cm. Tissue-cultured and mother plants exhibited clonal fidelity, similar metabolite and chlorophyll content, strong antioxidant activity, and equal efficacy for inhibiting -amylase and -glucosidase. This method can propagate elite clones of and offer its improvement via genetic transformation.

中文翻译：

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从 Uraria picta (Jacq.) Desv. 的子叶节直接再生芽。 ex DC.，一种重要的 dashmula 药物植物，以及遗传保真度、代谢谱和抗糖尿病活性的评估


根用于多种草药产品“Dashmula”。将其用于制剂制备已耗尽其可用性，导致药物掺假。子叶节的直接再生芽有望成为原材料来源。本研究旨在开发再生方案并验证其遗传和代谢保真度。种子发芽率低、休眠期长、生活力差。在野外对根的利用对其在自然界中的可用性构成了威胁。幼苗衍生的子叶节在补充有 BAP (0.5–3mgL)、激动素 (0.5–3mgL)、噻苯隆 (0.01–1mgL) 和托普林 (0.1–4mgL) 的 B5 培养基上培养。为了解决再生芽中的水分过多问题，在高琼脂浓度培养基上培养子叶节。将微芽暴露于IBA溶液（50-800mgL）脉冲处理以生根。使用 ISSR 和 SCoT 标记评估组织培养植物的遗传保真度，同时使用 HRMS 研究代谢保真度。评估了微繁植物的叶绿素含量、抗氧化和抗糖尿病活性。使用 2mgLTopolin 实现了最高的芽再生频率，每个外植体最多可再生 6.57±0.278 个芽。芽被拉长，叶子扩大，并且水分过多。 BAP (2mgL) 每个外植体诱导最多 9.83±0.333 个芽芽。 BAP 导致外植体褐变、大量愈伤组织形成、矮化和芽体水分过多。较高的琼脂浓度（1%）可以缓解水分过多的情况。 IBA (400mgL) 诱导每个芽最多产生 2.18±0.090 个根，根长为 9.23±0.033cm。 组织培养植物和母本植物表现出克隆保真度、相似的代谢物和叶绿素含量、强抗氧化活性以及抑制α-淀粉酶和β-葡萄糖苷酶的相同功效。该方法可以繁殖优良克隆并通过遗传转化对其进行改进。