Skin injuries and wounds present significant clinical challenges, necessitating the development of advanced wound dressings for efficient wound healing and tissue regeneration. In this context, the advancement of hydrogels capable of counteracting the adverse effects arising from undesirable reactive oxygen species (ROS) is of significant importance. This study introduces a hybrid hydrogel with rapid photocuring and excellent conformability, tailored to ameliorate the hostile microenvironment of damaged skin tissues. The hybrid hydrogel, composed of photoresponsive Gelatin Methacryloyl (GelMA) and Molybdenum-based nanoclusters (MNC), exhibits physicochemical characteristics conductive to skin regeneration. In vitro studies demonstrated the cytocompatibility and ROS-responsive behavior of the MNC/GelMA hybrid hydrogels, confirming their ability to promote human dermal fibroblasts (HDF) functions. The incorporation of MNC into GelMA not only enhances HDF adhesion, proliferation, and migration but also shields against oxidative damage induced by hydrogen peroxide (H₂O₂). Notably, in vivo evaluation in murine full-thickness skin defects revealed that the application of hybrid hydrogel dressings led to reduced inflammation, accelerated wound closure, and enhanced collagen deposition in comparison to control groups. Significantly, this study introduced a convenient approach to develop in situ ROS-scavenging hydrogel dressings to accelerate the wound healing process without the need for exogenous cytokines or medications. We consider that the nanoengineering approach proposed herein offers potential possibilities for the development of therapeutic hydrogel dressings addressing various skin-related conditions.

中文翻译：

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多功能钼基纳米团簇工程明胶甲基丙烯酰作为原位光可交联混合水凝胶敷料，用于增强伤口愈合


皮肤损伤和伤口提出了重大的临床挑战，需要开发先进的伤口敷料以实现有效的伤口愈合和组织再生。在这种情况下，开发能够抵消不良活性氧（ROS）引起的不利影响的水凝胶具有重要意义。这项研究介绍了一种具有快速光固化和出色的适形性的混合水凝胶，专门用于改善受损皮肤组织的恶劣微环境。这种混合水凝胶由光响应明胶甲基丙烯酰基（GelMA）和钼基纳米团簇（MNC）组成，表现出有利于皮肤再生的物理化学特性。体外研究证明了 MNC/GelMA 混合水凝胶的细胞相容性和 ROS 响应行为，证实了它们促进人真皮成纤维细胞 (HDF) 功能的能力。将 MNC 掺入 GelMA 不仅可以增强 HDF 粘附、增殖和迁移，还可以防止过氧化氢 (H ₂ O ₂ ) 诱导的氧化损伤。值得注意的是，对小鼠全层皮肤缺损的体内评估表明，与对照组相比，混合水凝胶敷料的应用可减少炎症、加速伤口闭合并增强胶原蛋白沉积。值得注意的是，这项研究引入了一种便捷的方法来开发原位ROS清除水凝胶敷料，以加速伤口愈合过程，而不需要外源细胞因子或药物。我们认为本文提出的纳米工程方法为开发解决各种皮肤相关病症的治疗性水凝胶敷料提供了潜在的可能性。