Spatiotemporal regulation of gene expression by polycomb repressive complex 2 (PRC2) is critical for animal and plant development. The fertilization independent seed (FIS)-PRC2 complex functions specifically during plant reproduction from gametogenesis to seed development. After a double fertilization event, triploid endosperm proliferates early, followed by the growth of a diploid embryo, which replaces the endosperm in and many dicots. Key genes critical for endosperm proliferation such as and are activated after fertilization. Here we report that two MADS-box AGAMOUS-LIKE (AGL) proteins associate with the key endosperm proliferation loci and recruit the FIS-PRC2 repressive complex at 4–5 days after pollination (DAP). Interestingly, AGL9 and AGL15 only accumulate toward the end of endosperm proliferation at 4–5 DAP and promote the deposition of H3K27me3 marks at key endosperm proliferation loci. Disruption of and or overexpression of or significantly influence endosperm proliferation and cellularization. Genome-wide analysis with cleavage Under Targets and tagmentation (CUT&Tag) sequencing and RNA sequencing revealed the landscape of endosperm H3K27me3 marks and gene expression profiles in Col-0 and . CUT&Tag qPCR also demonstrated the occupancy of the two MADS-box proteins and FIS-PRC2 on a few representative target loci. Our studies suggest that MADS-box proteins could potentially recruit PRC2 to regulate many other developmental processes in plants or even in fungi and animals.

中文翻译：

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两种 MADS-box 蛋白 AGL9 和 AGL15 招募 FIS-PRC2 复合物来触发拟南芥从胚乳增殖到胚胎发育的相变


多梳抑制复合物 2 (PRC2) 对基因表达的时空调节对于动植物发育至关重要。受精独立种子 (FIS)-PRC2 复合物在植物从配子发生到种子发育的繁殖过程中专门发挥作用。双受精事件后，三倍体胚乳早期增殖，随后生长二倍体胚，并取代许多双子叶植物中的胚乳。对胚乳增殖至关重要的关键基因，例如 和 ，在受精后被激活。在这里，我们报告了两个 MADS-box AGAMOUS-LIKE (AGL) 蛋白与关键的胚乳增殖位点相关，并在授粉后 4-5 天 (DAP) 招募 FIS-PRC2 抑制复合物。有趣的是，AGL9 和 AGL15 仅在 DAP 4-5 个胚乳增殖末期积累，并促进 H3K27me3 标记在关键胚乳增殖位点的沉积。胚乳增殖和细胞化的破坏和/或过度表达或显着影响。通过目标下切割和标记 (CUT&Tag) 测序和 RNA 测序进行全基因组分析，揭示了 Col-0 和 . 中胚乳 H3K27me3 标记和基因表达谱的概况。 CUT&Tag qPCR 还证明了两个 MADS-box 蛋白和 FIS-PRC2 在几个代表性靶位点上的占据。我们的研究表明，MADS-box 蛋白可能会招募 PRC2 来调节植物甚至真菌和动物的许多其他发育过程。