has been developed into many important crops, including cabbage, kale, cauliflower, broccoli and so on. The genome and gene annotation of cabbage (cultivar JZS), a representative morphotype of , has been widely used as a common reference in biological research. Although its genome assembly has been updated twice, the current gene annotation still lacks information on untranslated regions (UTRs) and alternative splicing (AS). Here, we constructed a high-quality gene annotation (JZSv3) using a full-length transcriptome acquired by nanopore sequencing, yielding a total of 59 452 genes and 75 684 transcripts. Additionally, we re-analyzed the previously reported transcriptome data related to the development of different tissues and cold response using JZSv3 as a reference, and found that 3843 out of 11 908 differentially expressed genes (DEGs) underwent AS during the development of different tissues and 309 out of 903 cold-related genes underwent AS in response to cold stress. Meanwhile, we also identified many AS genes, including and , that displayed distinct expression patterns within variant transcripts of the same gene, highlighting the importance of JZSv3 as a pivotal reference for AS analysis. Overall, JZSv3 provides a valuable resource for exploring gene function, especially for obtaining a deeper understanding of AS regulation mechanisms.

中文翻译：

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甘蓝基因组注释的改进凸显了选择性剪接的重要性


已发展成为许多重要作物，包括卷心菜、羽衣甘蓝、花椰菜、西兰花等。具有代表性的形态型甘蓝（JZS）的基因组和基因注释已被广泛用作生物学研究的共同参考。尽管其基因组组装已更新两次，但目前的基因注释仍然缺乏非翻译区（UTR）和选择性剪接（AS）的信息。在这里，我们使用纳米孔测序获得的全长转录组构建了高质量的基因注释（JZSv3），总共产生了 59 452 个基因和 75 684 个转录本。此外，我们以JZSv3为参考，重新分析了之前报道的与不同组织发育和冷反应相关的转录组数据，发现11 908个差异表达基因（DEG）中有3843个在不同组织发育和冷反应过程中发生了AS。 903 个寒冷相关基因中有 309 个经历了 AS 来应对冷应激。同时，我们还鉴定了许多 AS 基因，包括 和 ，它们在同一基因的变异转录本中表现出不同的表达模式，凸显了 JZSv3 作为 AS 分析关键参考的重要性。总体而言，JZSv3 为探索基因功能，特别是深入了解 AS 调控机制提供了宝贵的资源。