The compositions and contents of soluble sugars highly determine the flavor and quality of flshy fruits. In the present study, we found that the overexpression of transcription factor localized on the nucleus enhanced sucrose concentration while decreased fructose and glucose concentration in transgenic apple calli and ripening tomato fruits. To comprehensively understand the effects of the on the content of various soluble sugars in apple and tomato fruits, enzyme activities and related essential genes associated with the sugar metabolism and transportation pathway in -overrexpressed apple and tomato lines were analyzed. The results indicated that the overexpression of upregulated sucrose phosphate synthase (SPS) activity and the gene expression levels of and sucrose transporter , which was conducive to a large accumulation of sucrose in fruit cells. Meanwhile, overexpression downregulated the activity of enzymes involved in sucrose decomposition including cell wall invertase (CWINV), sucrose synthase (SUSY) and the corresponding gene expressions, as well as inhibited the expression levels of hexose transporter (s) and tonoplast sugar transporter (s) that transport hexose into vacuoles, resulting in a reduced hexose level in apple calli and tomato fruit. These findings enrich our understanding of the metabolism and regulation of soluble sugars in apple fruits.

中文翻译：

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转录因子 MdWRKY126 的过度表达改变了苹果和番茄果实中可溶性糖的积累


可溶性糖的组成和含量很大程度上决定了果肉的风味和品质。在本研究中，我们发现，位于细胞核的转录因子的过度表达提高了转基因苹果愈伤组织和成熟番茄果实中的蔗糖浓度，同时降低了果糖和葡萄糖浓度。为了全面了解α对苹果和番茄果实中各种可溶性糖含量的影响，对过表达的苹果和番茄品系中与糖代谢和运输途径相关的酶活性和相关必需基因进行了分析。结果表明，过表达上调蔗糖磷酸合酶（SPS）活性和蔗糖转运蛋白基因表达水平，有利于果实细胞中蔗糖的大量积累。同时，过表达下调参与蔗糖分解的酶的活性，包括细胞壁转化酶（CWINV）、蔗糖合酶（SUSY）及相应基因的表达，并抑制己糖转运蛋白（s）和液泡膜糖转运蛋白（s）的表达水平。 ）将己糖运输到液泡中，导致苹果愈伤组织和番茄果实中的己糖水平降低。这些发现丰富了我们对苹果果实中可溶性糖的代谢和调节的理解。