BACKGROUNDDicer1 plays a crucial role in regulating the development and reproduction of insects. Knockout of Dicer1 causes pupal deformity, low eclosion and low fecundity in Plutella xylostella, but the mechanism behind this phenomenon is not clear. This study aims to identify differentially‐expressed genes and miRNAs in the Dicer1‐knockout strain (ΔPxDcr‐1) and assess their impact on the reproduction and development of P. xylostella.RESULTSThe knockout of Dicer1 affected the expression of genes including the adipokinetic hormone/corazonin‐related peptide receptor (PxACPR). The expression of PxACPR was upregulated, and the expression of miR‐8514‐5p was downregulated in ΔPxDcr‐1 of P. xylostella. The dual luciferase reporter assay and pull‐down assay showed that miR‐8514‐5p bound to PxACPR in vitro and in vivo. The expression profiles demonstrated a negative correlation between PxACPR mRNA and miR‐8514‐5p in different developmental stages of the wild‐type strain. Both the miR‐8514‐5p agomir and double‐stranded RNA of ACPR (dsPxACPR) injected into the pre‐pupae inhibited the mRNA level of PxACPR, causing high mortality and deformity of pupae, and low fecundity and hatching rate, which were consistent with the phenotype of ΔPxDcr‐1. The injection of miR‐8514‐5p antagomir caused a similar phenotype to the injection of miR‐8514‐5p agomir. Additionally, the injection of miR‐8514‐5p antagomir significantly rescued the phenotype caused by dsPxACPR.CONCLUSIONThese results indicate that miR‐8514‐5p affects the development and reproduction of P. xylostella by regulating PxACPR, and the homeostasis of PxACPR expression is essential for the development and reproduction of P. xylostella. © 2024 Society of Chemical Industry.

中文翻译：

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microRNA-8514-5p调节脂肪运动激素/corazonin相关肽受体影响小菜蛾的发育和繁殖


背景Dicer1在调节昆虫的发育和繁殖中发挥着至关重要的作用。 Dicer1的敲除会导致小菜蛾蛹畸形、羽化低下和繁殖力低下，但这种现象背后的机制尚不清楚。本研究旨在鉴定 Dicer1 敲除菌株 (ΔPxDcr-1) 中差异表达的基因和 miRNA，并评估它们对小菜蛾繁殖和发育的影响。 结果 Dicer1 敲除影响了包括脂肪运动激素 / 等基因的表达。考拉佐宁相关肽受体（PxACPR）。小菜蛾ΔPxDcr-1中PxACPR表达上调，miR-8514-5p表达下调。双荧光素酶报告基因测定和下拉测定表明，miR-8514-5p 在体外和体内均与 PxACPR 结合。表达谱显示野生型菌株不同发育阶段的 PxACPR mRNA 和 miR-8514-5p 之间呈负相关。注射到预蛹中的 miR-8514-5p agomir 和 ACPR 双链 RNA (dsPxACPR) 均抑制 PxACPR 的 mRNA 水平，导致蛹高死亡率和畸形，以及低产卵率和孵化率，这与ΔPxDcr-1 的表型。注射 miR-8514-5p antagomir 引起与注射 miR-8514-5p agomir 相似的表型。此外，注射miR-8514-5p antagomir可显着挽救dsPxACPR引起的表型。结论这些结果表明，miR-8514-5p通过调节PxACPR影响小菜蛾的发育和繁殖，并且PxACPR表达的稳态对于小菜蛾的发育和繁殖至关重要。小菜蛾的发育和繁殖。 © 2024 化学工业协会。