In this study, a novel array electrospinning collector was devised to generate two distinct regenerated silk fibroin (SF) fibrous membranes: ordered and disordered. Leveraging electrostatic forces during the electrospinning process allowed precise control over the orientation of SF fiber, resulting in the creation of membranes comprising both aligned and randomly arranged fiber layers. This innovative approach resulted in the development of large‐area membranes featuring exceptional stability due to their alternating patterned structure, achievable through expansion using the collector, and improving the aligned fiber membrane mechanical properties. The study delved into exploring the potential of these membranes in augmenting wound healing efficiency. Conducting in vitro toxicity assays with adipose tissue‐derived mesenchymal stem cells (AD‐MSCs) and normal human dermal fibroblasts (NHDFs) confirmed the biocompatibility of the SF membranes. We use dual perspectives on exploring the effects of different conditioned mediums produced by cells and structural cues of materials on NHDFs migration. The nanofibers providing the microenvironment can directly guide NHDFs migration and also affect the AD‐MSCs and NHDFs paracrine effects, which can improve the chemotaxis of NHDFs migration. The ordered membrane, in particular, exhibited pronounced effectiveness in guiding directional cell migration. This research underscores the revelation that customizable microenvironments facilitated by SF membranes optimize the paracrine products of mesenchymal stem cells and offer valuable physical cues, presenting novel prospects for enhancing wound healing efficiency.

中文翻译：

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通过高产静电纺丝方法定制丝素蛋白纤维结构，以实现快速伤口愈合的可能性


在这项研究中，设计了一种新型阵列静电纺丝收集器来产生两种不同的再生丝素蛋白（SF）纤维膜：有序和无序。在静电纺丝过程中利用静电力可以精确控制 SF 纤维的方向，从而形成包含对齐和随机排列的纤维层的膜。这种创新方法导致了大面积膜的开发，由于其交替的图案结构而具有卓越的稳定性，可以通过使用收集器进行扩展来实现，并提高对齐的纤维膜的机械性能。该研究深入探讨了这些膜在提高伤口愈合效率方面的潜力。用脂肪组织源性间充质干细胞（AD-MSC）和正常人真皮成纤维细胞（NHDF）进行体外毒性测定，证实了 SF 膜的生物相容性。我们使用双重视角来探索细胞产生的不同条件培养基和材料的结构线索对 NHDF 迁移的影响。纳米纤维提供的微环境可以直接引导NHDFs迁移，同时影响AD-MSCs和NHDFs的旁分泌效应，从而提高NHDFs迁移的趋化性。特别是，有序膜在引导细胞定向迁移方面表现出显着的有效性。这项研究强调了SF膜促进的可定制微环境优化了间充质干细胞的旁分泌产物并提供了有价值的物理线索，为提高伤口愈合效率提供了新的前景。