Heat shock transcription factors (HSFs) are crucial regulators in the responses of plants to heat stress, but their mechanisms of action in trees are unclear. We identified an gene, , in × ( × ), which was highly responsive to heat. Its protein product was in both the cytoplasm and the nucleus. Stably overexpressing in poplar enhanced heat tolerance, improved photosynthetic capacity, increased antioxidant enzyme activities, reduced reactive oxygen species (ROS) levels, and upregulated antioxidant-related genes such as , , , , and under heat stress. Conversely, suppressing the gene by Virus-induced gene silencing (VIGS) in × decreased its heat tolerance. qRT-PCR analysis demonstrated that enhances the expression of several heat-protective genes, including small heat-shock protein genes (, , , , , , and ) and . Transcriptomic analysis suggested that the activation of glutathione metabolic pathways contributes to PeHSFA3-mediated heat tolerance. Our findings reveal that mediates resistance to heat stress in poplar and could be used to enhance the thermotolerance of poplar.

中文翻译：

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PeHSFA3对于欧美杨的热应激反应至关重要


热休克转录因子（HSF）是植物应对热胁迫的关键调节因子，但它们在树木中的作用机制尚不清楚。我们在 × ( × ) 中发现了一个基因 ，它对热高度敏感。其蛋白质产物存在于细胞质和细胞核中。在杨树中稳定过表达可增强耐热性，提高光合能力，增加抗氧化酶活性，降低活性氧（ROS）水平，并在热应激下上调抗氧化相关基因，如 、 、 、 、 和 。相反，在×中通过病毒诱导基因沉默（VIGS）抑制该基因会降低其耐热性。 qRT-PCR 分析表明，它增强了几种热保护基因的表达，包括小热休克蛋白基因 (, , , , , , 和 ) 和 。转录组分析表明，谷胱甘肽代谢途径的激活有助于 PeHSFA3 介导的耐热性。我们的研究结果表明，它介导杨树对热应激的抵抗力，可用于增强杨树的耐热性。