The cucurbitane-type triterpenoids of Linn. have blood lipid lowering effect and thereby improving non-alcoholic fatty liver. In this study, microwave-assisted extraction efficiently obtained these compounds which were further confirmed by the use of UPLC-MS/MS for target analysis and LC-HRMS for non-target analysis, combining GNPS techniques. The results indicated that the content of cucurbitane-type triterpenoids in bitter melon Hualien No. 5 was the highest among the others. Except for the immature whole fruit, the other plant parts such as fruits, seeds, vines and the whole plant of Hualien No. 5 with different maturity levels of immature, mature and overripe can significantly inhibit lipid accumulation in hepatocytes induced by oleic acid. The vines were proven as the most significant among the others where the active components for improving non-alcoholic fatty liver were rapidly screened and identified as 3β,7β,25-trihydroxycucurbita-5,23 ()-dien-19-al and Momordicine II through non-target analysis, multivariate analysis and molecular network.

中文翻译：

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利用分子网络策略进行靶标和非靶标分析，鉴定苦瓜中缓解非酒精性脂肪肝的潜在指标化合物


Linn 的葫芦烷型三萜类化合物。具有降血脂作用，从而改善非酒精性脂肪肝。在本研究中，微波辅助萃取有效地获得了这些化合物，并通过使用UPLC-MS/MS进行目标分析和LC-HRMS进行非目标分析，结合GNPS技术进一步证实了这些化合物。结果表明，花莲5号苦瓜中葫芦烷型三萜类化合物的含量最高。除未成熟全果外，花莲5号的果实、种子、藤蔓及未成熟、成熟、过熟不同成熟度的全株等植物部分均能显着抑制油酸诱导的肝细胞脂质积累。藤蔓被证明是其中最显着的，其中改善非酒精性脂肪肝的活性成分被快速筛选并鉴定为3β,7β,25-triHydroxycucurbita-5,23()-dien-19-al和Momordicine II通过非目标分析、多变量分析和分子网络。