Benzotriazole ultraviolet stabilizers (BUVSs) are a group of widely used chemicals added to a variety of consumer (e.g., plastics) and industrial (e.g., metal coating) goods. Although detected globally as an environmentally persistent pollutant, BUVSs have received relatively little toxicological attention and only recently have been acknowledged to affect development and the endocrine system in vivo. In our previous study, altered behavior, indicative of potential neurotoxicity, was observed among rainbow trout alevins (day 14 posthatching) that were microinjected as embryos with a single environmentally relevant dose of 2,4‐di‐tert‐butyl–6‐(5‐chloro‐2H‐benzotriazol‐2‐yl) phenol (UV‐327). In the present follow‐up study, we performed whole‐transcriptome profiling (RNA sequencing) of newly hatched alevins from the same batch. The primary aim was to identify biomarkers related to behavior and neurology. Dose‐specifically, 1 to 176 differentially expressed genes (DEGs) were identified. In the group presenting altered behavior (273.4 ng g−1), 176 DEGs were identified, yet only a fraction was related to neurological functions, including water, calcium, and potassium homeostasis; acetylcholine transmission and signaling; as well insulin and energy metabolism. The second objective was to estimate the transcriptomic point of departure (tPOD) and assess if point estimate(s) are protective of altered behavior. A tPOD was established at 35 to 94 ng UV‐327 g−1 egg, making this tPOD protective of behavioral alterations. Holistically, these transcriptomic alterations provide a foundation for future research on how BUVSs can influence rainbow trout alevin development, while providing support to the hypothesis that UV‐327 can influence neurogenesis and subsequent behavioral endpoints. The exact structural and functional changes caused by embryonic exposure to UV‐327 remain enigmatic and will require extensive investigation before being deciphered and understood toxicologically. Environ Toxicol Chem 2024;00:1–12. © 2024 The Author(s). Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

中文翻译：

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远离起源：2,4-二叔丁基-6-(5-氯-2H-苯并三唑-2-基)苯酚如何影响虹鳟鱼Alevins的转录组学研究


苯并三唑紫外线稳定剂 (BUVS) 是一组广泛使用的化学品，添加到各种消费品（例如塑料）和工业（例如金属涂层）产品中。尽管BUVS在全球范围内被检测为一种环境持久性污染物，但其毒理学关注相对较少，直到最近才被承认会影响体内发育和内分泌系统。在我们之前的研究中，在虹鳟鱼幼鱼（孵化后第 14 天）中观察到行为改变，表明潜在的神经毒性，这些鱼在胚胎时被显微注射了单一环境相关剂量的 2,4-二叔丁基-6-(5 -氯-2H-苯并三唑-2-基)苯酚(UV-327)。在本次后续研究中，我们对同一批次新孵化​​的鱼卵蛋白进行了全转录组分析（RNA 测序）。主要目的是识别与行为和神经病学相关的生物标志物。特定剂量下，鉴定出 1 至 176 个差异表达基因 (DEG)。在表现出行为改变的组中（273.4 ng g −1 ），鉴定出 176 个 DEG，但只有一小部分与神经功能相关，包括水、钙和钾稳态；乙酰胆碱传输和信号传导；以及胰岛素和能量代谢。第二个目标是估计转录组出发点（tPOD）并评估点估计是否可以保护行为改变。 tPOD 确定为 35 至 94 ng UV-327 g −1蛋，使 tPOD 能够保护行为改变。 总体而言，这些转录组改变为未来研究 BUVS 如何影响虹鳟鱼 alevin 发育奠定了基础，同时为 UV-327 可以影响神经发生和随后的行为终点的假设提供了支持。胚胎暴露于 UV-327 引起的确切结构和功能变化仍然是个谜，需要进行广泛的研究才能从毒理学角度破译和理解。环境毒理化学2024；00：1–12。 © 2024 作者。环境毒理学和化学由 Wiley periodicals LLC 代表 SETAC 出版。