This study investigates the efficacy of pyrite in enhancing biohydrogen production from xylose at low temperature (20 °C). Higher hydrogen yield rates () and reduced lag time () were achieved across initial xylose concentrations ranging from 2-10 g/L. At an optimal xylose concentration of 5 g/L, pyrite reduced by 2.5 h and increased from 1.3 to 2.7 mL h. These improvements are attributed to pyrite’s ability to enhance the secretion of extracellular polymeric substance and flavins, facilitate NADH and NAD generation and transition, and favor biohydrogen production. Thermodynamic analyses and Gibbs free energy calculations further elucidated pyrite’s role in the full reaction process and rate-limiting steps at low temperature. This study offers valuable insights into improving the efficiency of biohydrogen production at low temperature, with significant implications for energy conservation.

中文翻译：

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使用天然 FeS2 矿石在低温 (20 °C) 下提高木糖的生物氢产量：热力学分析和机理见解


本研究研究了黄铁矿在低温（20°C）下增强木糖生物氢生产的功效。在 2-10 g/L 的初始木糖浓度范围内，实现了更高的氢产率 () 和减少的滞后时间 ()。在最佳木糖浓度为 5 g/L 时，黄铁矿减少了 2.5 h，并从 1.3 mL h 增加到 2.7 mL h。这些改进归因于黄铁矿能够增强细胞外聚合物和黄素的分泌，促进 NADH 和 NAD 的生成和转化，并有利于生物氢的产生。热力学分析和吉布斯自由能计算进一步阐明了黄铁矿在低温全反应过程和限速步骤中的作用。这项研究为提高低温下生物制氢的效率提供了宝贵的见解，对节能具有重大意义。