Antifungal Activity and Mechanisms of 2-Ethylhexanol, a Volatile Organic Compound Produced by Stenotrophomonas sp. NAU1697, against Fusarium oxysporum f. sp. cucumerinum,Journal of Agricultural and Food Chemistry

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Antifungal Activity and Mechanisms of 2-Ethylhexanol, a Volatile Organic Compound Produced by Stenotrophomonas sp. NAU1697, against Fusarium oxysporum f. sp. cucumerinum
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-06-25 , DOI: 10.1021/acs.jafc.3c09851
Yu Zhang ₁ , Zeyuan Li ₁ , Shuxin Wei ₁ , Changsheng Xu ₁ , Minhua Chen ₁ , Jierong Sang ₁ , Yiru Han ₁ , Huang Yan ₁ , Zhoukun Li ₁ , Zhongli Cui ₁ , Xianfeng Ye ₁

Affiliation

Researchers often consider microorganisms from Stenotrophomonas sp. to be beneficial for plants. In this study, the biocidal effects and action mechanisms of volatile organic compounds (VOCs) produced by Stenotrophomonas sp. NAU1697 were investigated. The mycelial growth and spore germination of Fusarium oxysporum f. sp. cucumerinum (FOC), which is a pathogen responsible for cucumber wilt disease, were significantly inhibited by VOCs emitted from NAU1697. Among the VOCs, 33 were identified, 11 of which were investigated for their antifungal properties. Among the tested compounds, 2-ethylhexanol exhibited the highest antifungal activity toward FOC, with a minimum inhibitory volume (MIV) of 3.0 μL/plate (equal to 35.7 mg/L). Damage to the hyphal cell wall and cell membrane integrity caused a decrease in the ergosterol content and a burst of reactive oxygen species (ROS) after 2-ethylhexanol treatment. DNA damage, which is indicative of apoptosis-like cell death, was monitored in 2-ethylhexanol-treated FOC cells by using micro-FTIR analysis. Furthermore, the activities of mitochondrial dehydrogenases and mitochondrial respiratory chain complex III in 2-ethylhexanol-treated FOC cells were significantly decreased. The transcription levels of genes associated with redox reactions and the cell wall integrity (CWI) pathway were significantly upregulated, thus indicating that stress was caused by 2-ethylhexanol. The findings of this research provide a new avenue for the sustainable management of soil-borne plant fungal diseases.

中文翻译：

寡养单胞菌产生的挥发性有机化合物 2-乙基己醇的抗真菌活性和机制。 NAU1697，针对尖孢镰刀菌 f。 sp。黄瓜

研究人员经常考虑来自寡养单胞菌的微生物。对植物有益。在本研究中，寡养单胞菌产生的挥发性有机化合物（VOC）的杀菌效果和作用机制。对NAU1697进行了调查。尖镰孢菌丝生长和孢子萌发。 sp。 NAU1697 排放的 VOC 显着抑制了黄瓜枯萎病的病原体黄瓜(FOC)。在 VOC 中，鉴定出了 33 种，其中 11 种的抗真菌特性得到了研究。在测试的化合物中，2-乙基己醇对 FOC 表现出最高的抗真菌活性，最小抑制体积 (MIV) 为 3.0 μL/板（相当于 35.7 mg/L）。 2-乙基己醇处理后，菌丝细胞壁和细胞膜完整性受损导致麦角甾醇含量下降和活性氧（ROS）激增。通过使用微 FTIR 分析，在 2-乙基己醇处理的 FOC 细胞中监测 DNA 损伤，这表明细胞凋亡样细胞死亡。此外，2-乙基己醇处理的 FOC 细胞中线粒体脱氢酶和线粒体呼吸链复合物 III 的活性显着降低。与氧化还原反应和细胞壁完整性（CWI）途径相关的基因的转录水平显着上调，从而表明应激是由2-乙基己醇引起的。这项研究的结果为土传植物真菌病害的可持续管理提供了新途径。

更新日期：2024-06-25

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