BackgroundBacterial‐induced inflammation instigates the destruction of hard and soft tissues surrounding teeth in periodontitis. In severe cases, the increased number and activity of osteoclasts induces the resorption of alveolar bones, ultimately leading to tooth loss. Because of their diverse chemical structures and bioactivities, natural compounds are often suggested to treat a wide variety of diseases, including inflammatory disorders.MethodsIn the present study, we demonstrated an inhibitory effect of gossypetin, a hexahydroxy flavone, on osteoclast differentiation and bone resorption using in vitro culture of osteoclasts from mouse bone marrow macrophage (BMM) precursors and in vivo model of ligature‐induced periodontitis in mice.ResultsGossypetin significantly reduced the differentiation of osteoclasts from mouse BMM precursors in the presence of the receptor activator of nuclear factor κB ligand (RANKL). In vitro, gossypetin inhibited critical signaling events downstream of RANKL including the auto‐amplification of nuclear factor of activated T‐cells, cytoplasmic 1, Ca2+ oscillations, and the generation of reactive oxygen species. In a mouse ligature‐induced periodontitis model, the administration of gossypetin significantly reduced osteoclastogenesis and alveolar bone resorption. Furthermore, gossypetin prevented the ligature‐induced increase in macrophages and T cells and reduced the production of tumor necrosis factor‐α and interleukin‐6.ConclusionTaken together, these results show anti‐osteoclastogenic and anti‐inflammatory effects of gossypetin, suggesting the potential use of this natural compound in periodontitis.

中文翻译：

---

类黄酮棉酚可保护牙槽骨并限制结扎引起的小鼠牙周炎的炎症


背景细菌引起的炎症会导致牙周炎中牙齿周围硬组织和软组织的破坏。在严重的情况下，破骨细胞数量和活性的增加会引起牙槽骨的吸收，最终导致牙齿脱落。由于其不同的化学结构和生物活性，天然化合物通常被认为可以治疗多种疾病，包括炎症性疾病。 方法在本研究中，我们使用六羟基黄酮棉素证明了对破骨细胞分化和骨吸收的抑制作用小鼠骨髓巨噬细胞（BMM）前体破骨细胞的体外培养和小鼠结扎诱导牙周炎的体内模型。结果在核因子κB配体受体激活剂存在的情况下，棉素显着降低了小鼠BMM前体破骨细胞的分化（兰克）。在体外，棉素抑制 RANKL 下游的关键信号转导事件，包括活化 T 细胞核因子、细胞质 1、Ca 的自动扩增2+振荡和活性氧的产生。在小鼠结扎诱导的牙周炎模型中，给予棉布素可显着减少破骨细胞生成和牙槽骨吸收。此外，棉布素可防止结扎诱导的巨噬细胞和 T 细胞增加，并减少肿瘤坏死因子-α 和白细胞介素-6 的产生。结论综上所述，这些结果显示棉布素具有抗破骨细胞和抗炎作用，表明其潜在用途这种天然化合物在牙周炎中的作用。