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Microfluidic synthesis of hemin@ZIF-8 nanozyme with applications in cellular reactive oxygen species detection and anticancer drug screening
Lab on a Chip ( IF 6.1 ) Pub Date : 2024-06-24 , DOI: 10.1039/d4lc00290c Yanping Wang 1 , Shujun Feng 2 , Xuyuan Wang 2 , Chungui Tao 1 , Yuta Liu 2 , Yanyi Wang 2 , Yanfeng Gao 1, 2 , Jinsong Zhao 1 , Yujun Song 2
Lab on a Chip ( IF 6.1 ) Pub Date : 2024-06-24 , DOI: 10.1039/d4lc00290c Yanping Wang 1 , Shujun Feng 2 , Xuyuan Wang 2 , Chungui Tao 1 , Yuta Liu 2 , Yanyi Wang 2 , Yanfeng Gao 1, 2 , Jinsong Zhao 1 , Yujun Song 2
Affiliation
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Zeolitic imidazolate framework-8 (ZIF-8) encapsulating enzymatically active biomolecules has emerged as a novel biocompatible nanozyme and offers significant implications for bioanalysis of various biomarkers towards early diagnosis of severe diseases such as cancers. However, the rapid, continuous and scalable synthesis of these nanozymes still remains challenging. In this work, we proposed a novel microfluidic approach for rapid and continuous synthesis of hemin@ZIF-8 nanozyme. By employing a distinctive combination of zigzag-shaped channel and spiral channel with sudden expansion structures, we have enhanced the mixing efficiency within the chip and achieved effective encapsulation of hemin in ZIF-8. The resulting hemin@ZIF-8 nanoparticles exhibit peroxidase-like activity and are capable of detecting free H2O2 with a limit of detection (LOD) as low as 45 nM, as well as H2O2 secreted by viable cells with a detection threshold of approximately 10 cells per mL. By leveraging this method, we achieved successful detection of cancer cells and effective screening of anticancer drugs that induce oxidative stress injury in cancer cells. This innovative microfluidic strategy offers a new avenue for synthesizing functional nanocomposites to facilitate the development of next-generation diagnostic tools for early disease detection and personalized medicine.
中文翻译：
微流控合成hemin@ZIF-8纳米酶及其在细胞活性氧检测和抗癌药物筛选中的应用
封装酶活性生物分子的沸石咪唑酯框架 8 (ZIF-8) 已成为一种新型生物相容性纳米酶，对各种生物标志物的生物分析以及癌症等严重疾病的早期诊断具有重要意义。然而，这些纳米酶的快速、连续和可扩展的合成仍然具有挑战性。在这项工作中，我们提出了一种新颖的微流体方法，用于快速连续合成hemin@ZIF-8纳米酶。通过采用锯齿形通道和螺旋通道与突然膨胀结构的独特组合，我们提高了芯片内的混合效率，并实现了 ZIF-8 中氯化血红素的有效封装。由此产生的hemin@ZIF-8纳米颗粒表现出类似过氧化物酶的活性，能够检测检测限（LOD）低至45 nM的游离H 2 O 2 ，以及活细胞分泌的H 2 O 2检测阈值约为每毫升 10 个细胞。利用该方法，我们成功检测了癌细胞，并有效筛选了诱导癌细胞氧化应激损伤的抗癌药物。这种创新的微流体策略为合成功能性纳米复合材料提供了新途径，以促进用于早期疾病检测和个性化医疗的下一代诊断工具的开发。
更新日期：2024-06-28
中文翻译：
微流控合成hemin@ZIF-8纳米酶及其在细胞活性氧检测和抗癌药物筛选中的应用
封装酶活性生物分子的沸石咪唑酯框架 8 (ZIF-8) 已成为一种新型生物相容性纳米酶，对各种生物标志物的生物分析以及癌症等严重疾病的早期诊断具有重要意义。然而，这些纳米酶的快速、连续和可扩展的合成仍然具有挑战性。在这项工作中，我们提出了一种新颖的微流体方法，用于快速连续合成hemin@ZIF-8纳米酶。通过采用锯齿形通道和螺旋通道与突然膨胀结构的独特组合，我们提高了芯片内的混合效率，并实现了 ZIF-8 中氯化血红素的有效封装。由此产生的hemin@ZIF-8纳米颗粒表现出类似过氧化物酶的活性，能够检测检测限（LOD）低至45 nM的游离H 2 O 2 ，以及活细胞分泌的H 2 O 2检测阈值约为每毫升 10 个细胞。利用该方法，我们成功检测了癌细胞，并有效筛选了诱导癌细胞氧化应激损伤的抗癌药物。这种创新的微流体策略为合成功能性纳米复合材料提供了新途径，以促进用于早期疾病检测和个性化医疗的下一代诊断工具的开发。
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